沙棘油口服液对运动训练小鼠肝脏组织自由基代谢和超微结构影响的实验研究

Effects of Hippophae rhamnoides oil on metabolism of free radicals and ultrastructure of liver in trained mice

通过测定运动小鼠大脑超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量,观察其超微结构变化,探讨服用沙棘油对小鼠能力影响的生物化学机制。为此选用70只雄性ICR小鼠随机分为六组:安静对照组、安静沙棘组、训练即刻组、训练即刻沙棘组、训练恢复24h组和训练恢复24h沙棘组。经过6周(42d)游泳耐力训练后进行一次力竭性游泳,分析比较了各组小鼠血清谷草转氨酶(GFT)、肝脏超氧化物歧化酶(SOD))活性、丙二醛(MDA)含量等指标,同时还对各组肝脏细胞超微结构进行了电镜观察。结果:1)以上各组GPT除安静对照组与相应沙棘组和训练即刻组与训练恢复组间无显著差异外(P>0.05),其余各组间均有显著差异(P<0.05);2)SOD除安静对照组与相应沙棘组,训练即刻沙棘组与训练恢复沙棘组间无显著差异外(P>0.05),其余各组间均有显著差异(P<0.05);3)MDA除训练即刻组与相应沙棘组及恢复组间无显著差异外(P>0.05),其余各组间均有显著差异(P<0.05);4)沙棘组肝脏细胞中线粒体、肝浆网、肌丝等结构均比相应对照组完整,病变程度比对照组轻。结果表明,沙棘油能改善运动对肝脏细胞的损伤,提高肝脏组织抗自由基氧化的能力,同时还对肝脏细胞超微结构具有明显保护作用。

To study the effects of oil of Hippophae rhamnoides (OHR) supplements on sGPT, metabolism of free radical and ultrastructure of liver in trained mice, 70 male ICR mice were randomly divided into six groups : sedentary control group (SCG), sedentary+OHR group(SHG), immediately after exhaustive exercise in control group (IEC) and in OHR group (IEO), 24-hours recovery after exhaustive exercise in control group (REC) and in OHR group (REO). After 6-w swimming program with gradually increasing intensity, these mice carried out an exhaustive swimming before sacrifice. Serum GPT, SOD and MDA in myocardium were measured and the changes of ultrastructure of myocardium were observed by transmission electron microscope (TEM). Results indicated that: 1) serum GPT was significantly different among these groups except between SCG and SHG, IEC and REC; 2) SOD was significantly different among the above groups except between SCG and SHG, IEO and REO; 3) the concentration of MDA was significantly different among the above groups except between IEC and IEO, REC and REO; 4) the integrity of mitochondrion, rough-surfaced endoplasmic reticulum was better in OHR groups than in control groups and the pathological tendency of these ultra-structures was lighter in OHR groups compared with in control groups. OHR supplementation can reduce the damage of liver caused by exercise, enhance the antioxidative status of mice and protect the ultrastructure of liver.