水稻化感生物测试方法的比较及应用

Evaluation efficiency of different bioassay methods on allelopathic potential of Oryza sativa

选用 8个化感作用潜力各异的水稻品种 (系 ) :PI312 777、Lemont、Moroberekan、IAC2 5、IAC4 7、IAC12 0、Batatais、IguapeCateto为供试材料 ,以田间伴生稗草为受体材料 ,比较分析了目前较为通用的 3种生物测试方法 :琼脂迟播共培法 (RSA)、石英砂迟播共培法 (RSS)和根系分泌物培养法 (SRE)对水稻化感潜力的测试效果 .结果表明 ,RSA法应用于测试水稻化感作用潜力的效果最好 ,RSS法和SRE法的测试结果虽较为一致 ,但两者都存在不足 .据此 ,运用RSA法对 5 7个水稻材料进行了化感抗草种质资源的初步筛选 ,从中获得 5个强化感作用潜力的水稻材料 :IguapeCateto、PI312 777、Azucena、TaichungNative 1和IAC2 5 .

In this paper,three bioassay methods,i.e.,relay seeding in agar(RSA),relay seeding in silica(RSS) and seeding in rice root-exudation(SRE),were used to test the allelopathic potential of 8 rice cultivars on the target weed barnyardgrass(Echinochloa crusgalli).The results indicated that RSA was the ideal method for the bioassay,showing the highest efficiency in the evaluation of allelopathic potential.RSS and SRE had a lower efficiency than RSA,but these two methods showed the same tendency in evaluating the allelopathic potential of rice.RSA,the considered best bioassay method in this experiment,was used for 57 allelopathic rice germplasm screening,and 5 of them,i.e.,Iguape Cateto,PI312777,Azucena,Taichung Native 1 and IAC25 performed the strongest allelopathic potential in the suppression on barnyardgrass.