气升式光生物反应器培养海洋微藻的中试研究

Pilot scale research on the air-lift photobioreactor in the cultivation of marine microalgae

采用自行设计的600L气升式光生物反应器对两种海洋微藻湛江叉鞭金藻和盐藻进行了中试实验,重点研究了设备中安装内置光源和补充CO2对于微藻生长和氮、磷含量的影响。结果表明:不同光质的内部光源对微藻作用不同,叉鞭金藻在蓝光和红光下生长最好,盐藻生长最快为红光组和白光组,而且补充内置光源使两种微藻氮含量升高,但对藻体磷含量影响不大;补充高浓度CO2(700μL·L-1以上)能明显提高两种微藻生物量,并使藻体氮、磷含量有所增加。用该技术培养的微藻生长速度快,产量稳定,中试实验结果可为进一步推广应用奠定基础。

The effects of light quality and CO_2 concentration on the growth and the contents of nitrogen and phosphorus of two microalgae (Dicrateri.zhanjiangensis and Dunaliella sp.) in the 600 L air-lift photobioreactor systems that designed by oneself were investigated. The results showed that the effects of interior illuminant of different light qualities on microalgae were different. Dicrateri.zhanjiangensis and Dunaliella sp. both reached their maximum biomass in red, blue light and red, white light separately, and their nitrogen contents increased in the interior light sources, but all the interior illuminant had no effect on their phosphorus contents. By supplying high density of CO_2(above 700 μL·L^(-1)) in photoreactor, the biomass of two microalgae could increase apparently, and their nitrogen and phosphorus contents were significantly increased in high CO_2 concentration (above 700 (μL·L^(-1))) compared with low CO_2 concentration(350 μL·L^(-1)). The microalgae cultivated by using this technolege grown quickly and the output of which was steady. The result of pilot scale reasearch can provide groundwork for the popularization use of this technology.