利用RAPD标记对珙桐地理种群遗传分化的研究

Study on Genetic Differentiation for Geographic Population of Davidia involucrate by RAPD Marker

利用RAPD标记对珙桐全分布区5个种群的遗传多样性进行了分析。研究发现,珙桐具有丰富的遗传多样性,但由于小种群效应,以及缺乏有效的基因流,种群间遗传分化巨大,26%的遗传变异存在于种群间。使用Nei's遗传距离进行UPGMA聚类分析,可以很好地将珙桐划分为东南部和西北部两个种源区,为今后珙桐的种质资源保存和开发利用研究提供理论依据。

Davidia involucrate genetic diversity of 5 populations in distribution regions was analyzed by RAPD marker.It was found that D.involucrate possessed rich genetic diversity,however,the genetic differentiation was enormous among the populations,26% genetic variation existed among the populations due to small population effect and shortage of effective gene flow. By the use of UPGMA cluster analysis with Nei's genetic distance,D.involucrate could be soundly divided into two provenance plots of southeast and northwest,which would provide theory references for conservation and study on the development and application of germ plasm resource from D.involucrate.