牛蒡子提取物对糖尿病大鼠肾脏病变作用机制的实验研究

Research of Fructus Arctii and its extracts on the renal pathological changes of STZ-induced diabetic rats

目的 :研究牛蒡子及其提取物对链脲佐菌素 (STZ)糖尿病大鼠尿蛋白、尿微量白蛋白、肾脏TGF β 1mRNA和MCP 1mRNA表达的影响。方法 :以链脲佐菌素 (STZ)诱导的糖尿病大鼠为动物模型 ,中药治疗组分别给予牛蒡子粉饲料、牛蒡子水提物、醇提物进行 6周的实验 ,观察了大鼠的一般状况 ,尿蛋白、尿微量白蛋白及肾功能变化 ,聚合酶链反应 (RT PCR)方法测定肾皮质TGFβ1、MCP 1mRNA的表达。结果 :牛蒡子提取物能明显改善STZ大鼠多饮、多食和消瘦 ,降低尿蛋白、尿微量白蛋白 ,减少肾组织转化生长因子 β1(TGF β1)mRNA、单核趋化蛋白 1(MCP 1)mRNA的表达。 结论 :牛蒡子提取物对糖尿病大鼠肾脏病变有一定的改善 ,其作用机制可能与降低尿蛋白、尿微量白蛋白及肾脏TGF β1和MCP 1mRNA的表达有关 ,研究表明

AIM: To investigate the influence of Fructus Arctii and its extracts to the renal pathological changes of diabetic rat and its protective mechansim METHODS: The rat model induced by 55mg/kg STZ once intraperitoneally was adoped as the diabetic nephropathy of early stage, the extract and the powder of Fructus Arctii were given to the diabetic rat for six weeks. We observed the changes of general condition, and examined the expressions of TGF-β 1 (transforming growth factor-β 1) mRNA and MCP-1mRNA (monocyte chemoattractant protein-1) by reverse transcription-polymerase chain reaction (RT-PCR). CONCLUSION: The ethanol extract of Fructus Arctii relieved renal pathological changes of diabetic rat. The mechanism perhaps was related to the reduction in the expression of MCP-1mRNA and TGF-β 1mRNA in kidney; The study proved that the ethanol extract of Fructus Arctii was more effective than its water extract and coarse powder.