棉田中捕食天敌对棉蚜捕食作用的血清学鉴定

SEIOLOGICAL IDENTIFICATION OF THE PREDATORS ON APHIS GOSSYPII(HOMOPTERA; APHIDIDAE)IN COTTON FIELDS

本文采用双向扩散法,对流免疫电泳及反向间接血凝试验对棉田中的25种捕食天敌共1247头进行了测试,结果表明,双向扩散法鉴定有8种天敌捕食棉蚜,即:3种瓢虫、2种草蛉、1种食蚜蝇和2种蜘蛛;对流免疫电泳有9种,即3种瓢虫,2种草蛉、1种食蚜蝇及3种蜘蛛;反向间接血凝试验有14种,即:4种瓢虫,2种革蛉、 1种食蚜蝇及5种蜘蛛。这三种方法的灵敏度不同,对流免疫电泳比双向扩散法高4倍。反向间接血凝试验比双向扩散法高128倍。

A total of 1247 predator specimens,representing 25 predacious species,collected form cotton fields in Wuhan district,were tested using three serological methods:double diffusion(DD),counter immunoelectrophoresis(CIE),and reverse passive haemagglutination assay(RPHA).Eight species were identified by DD,9 by CIE,and 14 by RPHA.The differ- ence of the results was due to the sensitivity of the methods.CIE was 4 times more sensitive than DD,while RPHA 128 times than DD.