乌苏里藜芦碱在大鼠的血液流变学作用

Haemorheological effect of veratrum nigrum L.Var. ussurience Nakai alkaloids in rats

[目的 ]研究乌苏里藜芦碱 (VnA)对大鼠血液粘度的影响。 [方法 ]正常和血淤大鼠ivVnA1 5 μg·kg- 1,30 μg·kg- 1和 4 5 μg·kg- 1后取血 ,以微机化血液比粘度仪测定全血比粘度、血浆比粘度和红细胞压积等血液流变学指标。 [结果 ]正常大鼠ivVnA后全血比粘度显著降低 ,低切变率时自 2 0 .87± 3.6 3(NS组 )降至 1 3.0 8± 2 .4 2 (VnA中剂量组 ,P <0 .0 1 )和 1 1 .89± 4 .77(VnA大剂量组 ,P <0 .0 1 ) ,高切变率时自 6 .1 4± 1 .0 5 (NS组 )降至 4 .71± 1 .0 3(VnA大剂量组 ,P <0 .0 5 )。血淤模型大鼠ivVnA同样导致全血比粘度的显著降低 ,在低切变率时中剂量组为 1 8.98± 3.36 ,大剂量组为 8.35± 3.34,而血淤模型组为 5 0 .81± 7.5 4,P <0 .0 1 ,在高切变率时 ,大剂量组为 8.32± 2 .74 ,而血淤模型组为 1 2 .5 7± 3.0 6 ,P <0 .0 5。VnA在大剂量时对血浆粘度也有一定降低作用(1 .4 6± 0 .0 3vs 1 .5 3± 0 .0 6 ,P <0 .0 5 ,正常大鼠 ;1 .5 7± 0 .1 2vs 1 .87± 0 .4 4,P <0 .0 1 ,血淤大鼠 )。对血细胞压积无显著影响。 [结论 ]静脉给予VnA对血粘度具有明显的降低作用 ,对低切变率全血粘度的降低尤为明显。

Objective] To investigate the effect of veratrum nigrum L .Var. ussurience Nakai alkaloids (VnA)on rat blood viscosity. [Methods] Normal and blood stasis model rats were administered intravenously VnA at doses of 15,30 and 45 μg·kg -1 .The blood samples were collected and tested for haemorheological parameters including whole blood viscosity(WBV), plasma viscosity and HCT by means of computer-aided viscometer.[Results] In normal rats the WBV at low shear rate(SR) was significantly reduced by iv VnA from 20.87±9.63(NS group) to 13.08±2.42(middle dose group, P <0.01) and 11.89±4.77(large dose group, P <0.01),at high SR from 6.14±1.05(NS group) to 4.71±1.03(large dose group, P <0.05).In blood stasis model rats, WBV was markedly reduced by iv administration at low SR (18.98±3.36 for middle dose and 8.35±3.34 for large dose vs 50.81±7.54 for blood stasis model) and at high SR(8.32±2.74 for large dose vs 12.57±3.06 for blood stasis model, P <0.05).It was found that VnA at large dose had some lowering effect on plasma viscosity(1.46±0.03 vs 1.53±0.06 and 1.57±0.12 vs 1.87±0.44 in normal and blood stasis model,respectively, P <0.05 and P <0.01),and no significant effect on HCT could be measured following administration of VnA. [Conclusion] VnA administered by iv has significant lowering effect on blood viscosity ,especially, on WBV at low SR,which is independent on number and volume of RBC.