摘要
经过常规处理后,剥取香蕉球茎顶端分生组织带1～2个叶原基的生长点作外植体,接种在改良MS和含适量BA、NAA.KT 2mg/L和椰乳0.1%的培养基上。经这样培养成的植株用ELISA和生物检测法进行检测都没有检出病毒。
After treated conventionally, the explants of meristem with 1～2 leave primordia were excised from the rhizome apex of the banana plant. Then, this material was inoculated on the modified MS medium with 0.1% coconut milk and KT 2mg/L, and suitable amounts of BA, NAA were added. The successfully grown plantlets were free from virus infections as tested by ELISA and biological determination.
关键词
香蕉
组织培养
再生植株
banana
shoot tip meristem
tissue culture
regenerative plantlet
virus removal
