卡波金和氧气对豚鼠耳蜗作用的比较

A comparative study on the effects of carbogen and oxygen on the cochlear ischmia among guinea pigs

目的 探讨卡波金和氧气对豚鼠缺血耳蜗作用的异同。方法 动物分为5组,即正常对照组、假手术组、缺血组、缺血后卡波金干预组、缺血后氧气干预组。每组20只,10只做耳蜗血流量测试,10只做ABR测试、耳蜗基底膜铺片。结果 耳蜗血流卡波金组较缺血组增加31.46％,氧气组较缺血组降低26.13％;听觉阈值卡波金组较缺血组降低5.5dBSPL,氧气组与缺血组之间无差异性,耳蜗底转毛细胞纤毛倒伏卡波金组较缺血组减轻,氧气组没有改变。结论 吸入卡波金使缺血耳蜗的血流量增加,吸入氧气使缺血耳蜗的血流量降低。卡波金可减轻缺血耳蜗形态和功能损伤,氧气对之没有改善作用。

Objective To be carried out was a comparative investigation on the effects of carbogen and oxygen on the cochlear ischemia among guinea pigs. Methods One hundred guinea pigs were randomly divided into 5 groups, i. e. bland controlling group (BCG), sham-operated group (SOG), ischemia modeling group (IMG), carbogen treating group (CTG) and oxygen treat- ing group(OTG), of the 20 animals in each group, 10 were taken to determine the blood flow of cochlea and 10 were to test ABR and to prepare the surface preparation samples of cochlea. Re- sults Compared with that of TMG, the blood of cochlea was increased by 31. 46% in CTG and decreased by 26. 13% in OTG, the hearing threshold was declined 5. 5dB SPL in CTG and was seen with no significant difference in OTG. Furthermore, the changes in the morphology of hair cells in the base turn of cochlea were less in CTG than that in IMG, while there was no signifi- cant difference seen in OTG. Conclusion Inhalation of carbogen can increase blood flow and im- prove the morphological and functional conditions in cochlear ischemia. In contrast, oxygen inhala- tion may induce further decrease of blood flow and give no improving effects on the morphological and functional conditions in the cochlear ischemia.