金属硫蛋白在肾母细胞瘤中表达状况分析

Metallothionein expression in nephroblastomas

目的 探讨金属硫蛋白 (metallothionein ,MT)在肾母细胞瘤中表达状况及术前化疗对其表达的影响。方法 本组病例 30例 ,其中术前化疗 12例 ,化疗 2周 10例 ,2周以上 2例。采用免疫组化检测技术对 30例肾母细胞瘤瘤体组织、10例瘤旁肾组织和 10例正常肾组织进行免疫组化染色 ,观察金属硫蛋白在各种组织中表达的状况。分析金属硫蛋白在肾母细胞瘤各组织类型中表达状况以及术前化疗对其表达的影响。结果 正常肾组织无MT表达 ,瘤旁肾组织镜下为正常肾组织 ,MT表达很强 ,瘤体组织较弱 ,相互比较差异有统计学意义 (P <0 .0 5 )。MT在瘤体各组织类型中的表达无明显差异 (P >0 .0 5 )。术前化疗组瘤体MT的表达高于术前化疗组 (P <0 .0 5 )。瘤体组织中MT阳性表达患儿预后优于阴性表达者。结论 肾母细胞瘤的发生对MT的表达可能有诱导机制 ,尤其对瘤体周围肾组织 ,而瘤体MT的表达可能不利于术前化疗 ,同时术前化疗可以诱导瘤体MT的表达。患儿预后与瘤体组织MT表达呈正相关。

Objective To examine the nephroblastoma (NB) metallothionein (MT) expression before and after chemotherapy.Methods Of 30 patients with NB,12 underwent preoperative chemotherapy (10 for 2 weeks and 2 for 4 weeks). Using the immunochemical technique,we studied the MT expressions in the normal kidney tissues、the nephroblastoma tissues and the tissues adjacent to nephroblastoma.Results No MT expression was detected on normal renal tissues. Weak MT staining was detected in nephroblastoma whereas the adjacent non-tumor renal tissue showed significantly stronger MT staining. The MT in nephroblastoma tissues after chemotherapy was stronger. The survival rate of MT positive patients was better than that of MT negative patients ( P < 0.05 ).Conclusions NB may induce MT expression. Chemotherapy may also induce MT expression nephroblastoma tissues. The prognosis positively correlates with MT expression levels.