期刊文献+

内皮素-1对黑素细胞黏附和迁移的影响(英文) 被引量:1

Effects of endothelin-1 on melanocyte adhesion and migration
下载PDF
导出
摘要 目的 :体外研究内皮素 1对黑素细胞黏附和迁移的作用。方法 :用内皮素 1(ET 1)处理人表皮纯化培养的黑素细胞 ,观察其在纤维连接素包裹的培养板上的黏附以及通过微孔滤膜的情况 ,并与干细胞因子及未加因子组作对照比较。结果 :与干细胞因子组相比 ,内皮素 1处理的黑素细胞更易黏附于培养板和通过微孔滤膜 ,尤其是当其浓度在 32nmol/L时 ,而它的浓度超过 12 8nmol/L时 ,对黑素细胞的黏附和迁移却呈现抑制作用。除了 2nmol/L组 ,其他任何浓度下内皮素 1均对黑素细胞黏附起促进作用 (P <0 .0 1)。但对黑素细胞迁移来说 ,每个观察浓度下的内皮素 1均表现为迁移促进作用 ,且这种作用强于干细胞因子处理的黑素细胞 (P <0 .0 1)。结论 :内皮素 1可以影响黑素细胞的黏附和迁移 ,这可以部分解释内皮素 1对白癜风皮损区黑素细胞的功能有调节作用。 Objective To investigate the effect of endothelin-1 (ET-1) on the adhesion and migration of melanocyte in vitro.Methods Human epidermal melanocytes that had been cultured and purified were treated with ET-1 and observed for adhesion to bovine serum fibronectin-coated culture dishes. Stem cell factor (SCF) and ET-1 treated cells were also examined for migration into micropore filters coated with the same protein. Results Compared with the SCF group, ET-1 treated melanocytes were easier to adhere to the dishes and to be moved into the filters, especially when the concentration was 32 nmol/L. When the concentration of ET-1 was 128 nmol/L or more, it could inhibit the adhesion and migration of melanocyte.At any concentration of ET-1 except at 2 nmol/L, there was a significant difference between ET-1-treated and untreated melanocytes (P<0.01) when the adhesion test was carried out.However, even at 2 nmol/L ET-1, there was obviously increased migration compared with those of ET-1 untreated melanocytes and SCF-treated cells (P<0.01).Conclusion ET-1 may influence the adhesion and migration of melanocyte, which can partly explain the capacity of ET-1 to regulate the melanocyte function in vitiligo lesions.
出处 《中南大学学报(医学版)》 CAS CSCD 北大核心 2004年第3期247-251,共5页 Journal of Central South University :Medical Science
基金 国家自然科学基金 (30 2 0 0 2 4 7)
关键词 内皮素-1 黑素细胞 细胞黏附 ET-1 白癜风 melanocyte endotehlin-1 adhesion migration
  • 相关文献

参考文献14

  • 1Kemp EH, Waterman EA, Gawkrodger DJ, et al. Molecular mapping of epitopes on melanocyte-specific protein Pmel17 which are recognized by autoantibodies in patients with vitiligo [J]. Clin Exp Immunol, 2001, 124 (3): 509-515.
  • 2Cui J, Shen LY, Wang GC. Role of hair follicles in the repigmentation of vitiligo [J]. J Invest Dermatol,1991,97 (3):410-416.
  • 3Kunisada T, Yoshida H, Yamazaki H, et al. Transgene expression of steel factor in the basal layer of epidermis promotes survival, proliferation, differentiation and migration of melanocyte precursors[J]. Development,1998,125 (15):2915-2923.
  • 4Neitmann M, Alexander M, Brinckmann J, et al. Attachment and chemotaxis of melanocytes after ultraviolet irradiation in vitro [J]. Br J Dermatol, 1999, 141 (5): 794-801.
  • 5Morelli JG, Yohn JJ, Zekman T, et al. Melanocyte movement in vitro: role of matrix proteins and integrin receptos [J]. J Invest Dermatol, 1993, 101 (4):605-608.
  • 6Eisinger M,Marko O. Selective proliferation of normal human melanocytes in vitro,in the presence of phorbol ester and cholera toxin[J]. Proc Natl Acad Sci,1982,79 (6):2018-2022.
  • 7Situ R,Inman DR, Fligiel SEG, et al. Effects of all-trans-retinoic acid on melanocyte adhesion and motility[J]. Dermatology,1993,186 (1):38-44.
  • 8Yee C,Thompson JA,Roche P,et al.Melanocyte destruction after antigen-specific immunotherapy of melanoma: Direct evidence of T cell-mediated vitiligo[J].J Exp Med,2000,192(11):1637-1644.
  • 9Njoo MD, Spuls PI, Bos JD, et al. Nonsurgical repigmentation therapies in vitiligo Meta-analysis of the literature [J]. Arch Dermatol, 1998,134 (12): 1532-1540.
  • 10Flabella R, Arrunategui A,Barona MI, et al.The minigrafting test for vitiligo: detection of stable lesions for melanocyte transplantation [J]. J Am Acad Dermatol, 1995,32 (2): 228-232.

同被引文献19

引证文献1

二级引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部