抗氧化剂An7845对K562白血病细胞的增殖抑制和凋亡诱导活性

Effects of an antioxidant (An7845) on the proliferation and apoptosis of K562 leukemia cells

目的 :观察抗氧化剂An784 5在体外对K5 6 2白血病细胞增殖及凋亡的影响。方法 :采用液体培养实验 ,MTT实验 ,集落培养实验观察抗氧化剂An784 5对K5 6 2细胞增殖的影响 ,采用细胞形态学及DNA片段凝胶电泳观察和检测细胞凋亡。结果 :不同浓度的An784 5 (5× 10 8～ 5× 10 5mol/L)对K5 6 2细胞具有抑制增殖的作用 ,并呈剂量依赖关系。经An784 5 (5× 10 7mol/L)处理后 ,K5 6 2细胞在形态学上可见明显凋亡细胞 ,DNA琼脂糖凝胶电泳谱可见DNA梯形带。结论 :抗氧化剂An784 5能抑制K5 6 2白血病细胞增殖和诱导其凋亡。

Objective To determine the effects of an antioxidant (An7845)on the proliferation inhibition and apoptosis induction of a human leukemia cell line (K562). Methods We evaluated the effects of An7845 on K562 cell proliferation by cellular enumeration in liquid culture assay, MTT assay and colony formation assay. We also verified the effects of An7845 on K562 cell apoptosis by cellular morphology and the DNA fragments appeared in agarose gel electrophoresis.Results The proliferation of K562 leukemia cells was inhibited by An7845(5×10 -8 ～5×10 -5 mol/L) in a dose-dependent manner. K562 cells were induced toward apoptosis by An7845 (5×10 -7 mol/L).Conclusion An7845 can effectively inhibit the proliferation and induce the apoptosis of K562 leukemia cells.