革兰阴性杆菌9种氨基糖苷类修饰酶基因的检测

Detection of aminoglycosides-modifying enzymes gene in gram-negative bacilli

目的 调查革兰阴性杆菌氨基糖苷类修饰酶 (AMEs)基因的流行状况。 方法 建立检测AMEs基因的PCR法 ,检测临床分离的 2 0株革兰阴性杆菌 9种AMEs基因。 结果 2 0株革兰阴性杆菌中 6株检出AMEs基因 (30 % ) ,同一菌株可产生 2种以上AMEs ,且不同菌株产生的AMEs有很大差异。 3株表型为氨基糖苷类药物敏感 ,但检出AMEs基因 ;8株表型为氨基糖苷类药物耐药 ,但 9种AMEs基因检测为阴性。本研究肺炎克雷伯菌的aac(3) Ⅱ、大肠埃希菌的aac(6′) Ⅰ、铜绿假单胞菌的aac(3) Ⅱ和ant(2″) Ⅰ基因已成功注册GenBank。 结论 产生AMEs是细菌对氨基糖苷类抗生素耐药的机制之一。不同菌株产生的AMEs有很大差异。

Objective To investigate the situation of prevalence concerning aminoglycosides-modifying enzymes(AMEs) gene in gram-negative bacilli. Methods PCR methods was established to detect the AMEs gene in 20 strains of gram-negative bacilli. Results Six of 20 strains gram-negative bacilli were positive for AMEs gene(30%) ;Two or more AMEs were produced by the same bacilli and the AMEs produced by different bacilli have great diversities, 3 strains of bacilli susceptive to aminoglycosides were positive for AMEs gene and 8 strains of bacilli resistant to aminoglycosides were negative. The nucleotide sequence of the AMEs[aac(3)-Ⅱ, aac (6' ) -Ⅰ, aac(3)- Ⅱ and ant(2”)-Ⅰ]described in this paper have been submitted to the GenBank sequence databases. Conclusion Production of AMEs is one of the essentianl mechanism of aminoglycosides-resistance. The AMEs produced by different bacilli have great diversities.