慢性乙型肝炎患者氧化损伤的研究

Oxidative stress in patients with chronic hepatitis B

目的 探讨慢性乙型肝炎 (乙肝 )患者体内氧化损伤的情况。方法 检测 30例慢性乙肝患者氧化损伤指标 (丙二醛、总抗氧化能力、抗坏血酸 )、肝功能、乙型肝炎病毒 (HBVDNA) ,并做出统计分析。结果 慢性乙肝患者组与正常对照组比较 :丙二醛浓度明显升高 (P <0 0 5 ) ;ALT正常组与正常对照组比较 :抗坏血酸血清浓度明显升高 (P <0 0 1) ;ALT异常组与正常对照组及ALT正常组比较 :丙二醛血清浓度均明显升高 (P <0 0 5 )。在慢性乙型肝炎患者中 ,丙二醛浓度与ALT水平呈明显正相关 (r=0 6 1) ,抗坏血酸浓度与ALT水平呈明显负相关 (r =- 0 6 4 )。乙肝HBVDNA与抗氧化指标间无联系 (P >0 0 5 )。总抗氧化能力指标在各组间比较均无明显改变。结论 乙肝患者体内有氧化 抗氧化功能障碍。慢性乙肝患者ALT升高时 ,体内氧化损伤程度加重。慢性乙型肝炎患者ALT正常者 ,体内氧化损伤程度不高。氧化损伤指标与HBVDNA是肝炎患者中相对独立的检查指标。

Objective To study oxidative stress in patients with chronic hepatitis B. Methods Malondialdehyde (MDA),total anti-oxidative ability and ascorbic acid were measured as markers of oxidative stress in 30 patients with chronic hepatitis B,besides HBV DNA and ALT. Results MDA was significantly higher in patients with hepatitis B than the controls ( P <0.05). Ascorbic acid was significantly higher in patients with normal ALT than the controls ( P <0.01). MDA was significantly higher in patients with increased ALT than the controls and in patients with normal ALT. MDA was significantly positively correlated with ALT ( r =0.61),and ascorbic acid was significantly negatively correlated with ALT ( r =-0.64) in patients with hepatitis B. No significant relationship was found between HBV DNA and other indices of oxidative stress. No significant difference in total anti-oxidative ability was found among all groups. Conclusion There was a disturbance between oxidative stress and anti-oxidative ability in patients with chronic hepatitis B. In patients with increased ALT,oxidative stress became high. In patients with normal ALT,oxidative stress level was low. The indices of oxidative stress should be detected in patients with hepatitis B,in addition to HBV markers.