儿童病毒性肺炎外周血细胞凋亡的研究

Apoptosis of peripheral blood cells of children with viral pneumonia

目的 通过对病毒性肺炎外周血细胞凋亡的研究 ,探讨病毒感染的致病机理和机体的免疫反应。方法 采集病毒性肺炎患儿急性期、恢复期新鲜外周抗凝血 ,用FACSCalibur流式细胞仪收获细胞并用CellQuest软件分析结果 ,分别得到外周血之中性粒细胞、淋巴细胞中的活细胞、早期凋亡细胞、晚期凋亡细胞、死亡细胞的百分数。肺炎组共 2 8例 ,平均年龄 (1 30± 1 16 )岁。对照组 2 4例 ,平均年龄 (1 82± 0 97)岁。计量资料以 x±s表示 ,肺炎组和对照组间做方差分析 ,肺炎急性期与恢复期间用配对t检验。结果 病毒性肺炎患儿急性期、恢复期外周血中性粒细胞、淋巴细胞中活细胞百分率较对照组明显低 ,二者之间差异有非常显著意义 (P <0 0 1)。早期凋亡细胞百分率均较对照组明显高 ,二者之间差异有显著意义 (P <0 0 5 )。而患儿急性期、恢复期比较仅淋巴细胞中活细胞、晚期凋亡细胞、死亡细胞百分率差异有显著意义 (P <0 0 5 ) ,其余各项差异均无统计学意义。结论 病毒性肺炎时外周血中性粒细胞、淋巴细胞凋亡增加 ,存活细胞减少 ,所以适当增加细胞凋亡的药物可能缩短病程。

Objective To study apoptosis of peripheral blood cells of children with viral pneumonia,explore immunopathogenesis and the possibility of immunotherapy of patients with viral pneumonia. Methods Fresh peripheral blood samples were collected from 28 patients with viral pneumonia and 24 healthy children were treated and run through the flow cytometry. The data were acquired using CellQuest software and the percentage of live cells,viable apoptotic cells,non-viable apoptotic cells and dead cells of neutrophils and lymphocytes were counted. The patients with viral pneumonia were hospitalized at our hospital. The average age of patients was 1.3 years;24 healthy children were served as control group (age 1.8 years on average). T-test and variance analysis by SPSS FOR WINDOWS 10.0 software was used for statistical analysis. Results The percentage of live neutrophils and lymphocytes in the acute stage and recovery stage in patients were significantly lower than that in control group ( P <0.01). The percentage of viable apoptotic neutrophils and lymphocytes in two stages in patients were significantly higher than that in control group( P <0.05).Except for the percentage of live cells, non-viable apoptotic cells and dead lymphocytes,others had no difference between the patients and control groups. Conclusion Apoptosis of neutrophils and lymphocytes of peripheral blood cells of children with viral pneumonia increased. Whereas the percentage of live cells decreased. Drugs that can accelerate apoptosis may be helpful in treatment of viral pneumonia.