定量酶联免疫吸附试验检测各种结缔组织病患者血清中的抗可提取核抗原抗体

Determination of the serum anti-extracted nuclear antigens autoantibodies in the patients with connective tissue disease by quantitative ELISA

目的 定量检测各种结缔组织疾病 (CTD)患者血清中抗可提取核抗原 (ENA)自身抗体滴度 ,并探索其临床意义。方法 自制抗各种ENA单克隆抗体 ,以夹心酶联免疫吸附试验 (ELISA)定量检测 1 2 8例CTD患者、5 0例非CTD患者及 6 0名健康人血清的各种抗ENA自身抗体滴度。用健康人血清各ENA抗体滴度的均数加上 3倍标准差 ( x +3s)为cutoff值 ,界定各种ENA抗体检出率。结果 与健康对照组和非CTD患者组比较 ,抗Sm抗体在系统性红斑狼疮 (SLE)、抗ul RNP抗体在SLE和混合性结缔组织病 (MCTD)、抗SSA和SSB在干燥综合征 (SS)、抗Scl 70抗体在系统性硬化病 (PSS)、抗Jo 1抗体在多发性肌炎 /皮肤炎 (PM/DM)患者中的水平和阳性率都明显升高 (P <0 .0 1 )。结论 定量检测CTD患者血清中各种ENA抗体滴度 ,对CTD疾病的鉴别。

Objective To study the clinical significance of the quantitative determination of the various anti extracted nuclear antigens(ENA) autoantibodies in the various connective tissue disease (CTD). Methods The levels of various anti ENA autoantibodies in the serum from 128 CTD patients, 50 non CTD patients and 60 healthy persons were determined by a quantitative sandwich ELISA kit. The prevalence of these antibodies were also determined in 60 healthy persons. Results High level and positive rate of anti Sm antibody were detected in systemic lupus erythematosus(SLE), anti ul RNP antibody in SLE and mixed connective tissue disease (MCTD) anti SSA and SSB antibody in Sjogren's syndrome (SS), anti Scl 70 antibody in progress systemic scleroderma(PSS), and anti Jo 1 antibody in polymyositis/dermatomyositis(PM/DM). The level and positive rate of these autoantibodies were significant higher than that of non CTD patients and healthy persons ( P <0.01). Conclusions The various anti ENA autoantibodies can be quantitatively detected by the quantitative sandwich ELISA kit and the detection of these anti ENA autoantibodies is useful for diagnosis of patients with various CTD.