中国6个群体线粒体DNA RegionⅤ的缺失多态性

Polymorphism of Deletion in Mitochondrial DNA RegionⅤAmong 6 Populations in China

【目的】研究中国广东地区汉族、华东地区汉族、内蒙古汉族、云南白族、内蒙古蒙古族、新疆维吾尔族等6个群体在线粒体DNARegionⅤ的多态性。【方法】PCR扩增后采用变性高效液相色谱技术分离片段,检测线粒体DNARegionⅤ9bp缺失的频率。【结果】在中国6个群体中发现标准型、缺失型和3型3种多态类型,9bp缺失频率在6个群体分别为21.4,18.0,14.0,16.9,7.0,10.4。【结论】中国6个群体在线粒体DNARegionⅤ均存在9bp缺失的多态性,6个群体间的缺失频率存在差异。

To investigate the polymorphism of mitochondrial DNA RegionⅤamong Guangdong Han, East China Han, Neimenggu Han, Yunnan Bai, Menggu, Weiwuer populations.Denaturing high performance liquid chromatography was used to analyze PCR products of mtDNA RegionⅤ.Three types of amplicon fragments were observed in 6 populations, including standard type, deletion type, and 3 repeat type. The frequencies of 9 bp deletion among 6 populations were 21.4, 18.0, 14.0,16.9,7.0,and 10.4,respectively.[Conclusion]The 9 bp deletion in mtDNA RegionⅤis discovered in all 6 populations and there is difference among the frequencies of 9 bp deletion of 6 populations.