维生素E酯预处理对小鼠原位脂肪肝移植的影响

Vitamin E succinate pretreatment effects on a fatty liver transplantation model

目的 探讨供者用维生素E酯 (VES)预处理对原位小鼠脂肪肝移植的保护作用及其机理。方法 建立小鼠原位肝移植模型。随机分为正常小鼠对照组 (n =8)、肥胖小鼠对照组 (n =8)、非脂肪肝移植组 (n =12 )、脂肪肝移植组 (n =12 )、VES预处理脂肪肝移植组 (n =12 )。术后检测各组血清丙氨酸转氨酶 (ALT)、天冬氨酸转氨酶 (AST)相对水平 ;肝脏组织HE染色 ,油红O染色脂肪定量 ,ATP含量及解偶联蛋白 2 (UCP 2 )水平。结果 VES预处理脂肪肝移植组移植后 2h存活率为4 5 % ,而脂肪肝移植组为 2 5 % ,差异有显著性 (P <0 .0 5 ) ;VES预处理脂肪肝移植组血清ALT与AST水平与ATP浓度分别为 (32 33± 10 6 5 )U/L、(4386± 92 4 )U/L、0 .19± 0 .0 1,而脂肪肝移植组分别为 (832 0± 4 6 5 5 )U/L、(6 4 94± 2 6 5 5 )U/L、0 .13± 0 .0 1,差异有显著性 (P <0 .0 5 ) ;VES预处理脂肪肝移植组肝细胞损伤程度明显减轻 ,线粒体UCP 2水平低于脂肪肝移植组。结论 维生素E酯预处理对小鼠脂肪肝移植有一定保护作用。

Objective To study the influence of Vitamin E succinate (VES) pretreatment on mouse orthotopic liver transplantation (OLTx).Methods Using the non-arterialized mouse OLTx model,size matched lean and ob/ob (steatosis) donor mice were transplanted into lean recipients. VES was administered intravenously at 40 000 U /kg 30 min before the operation in recipients and donors. Endpoints included survival,serum ALT and AST,hepatocyte injury by HE,intrahepatocyte lipid content via Oil-Red O staining,hepatocyte ATP and UCP-2 levels.Results The increased survival rate was observed on OLTx pretreated with VES ( 45 % ) as compared with OLTs ( 25 % )( P < 0.05 );The levels of ALT and AST and the concentration of ATP in VES pretreated OLTx were ( 3 233 ± 1 065 )U/L,( 4 386 ± 924 )U/L and 0.19 ± 0.01 respectively,but in OLTx were ( 8 320 ± 4 655 )U/L,( 6 494 ± 2 655 )U/L and 0.13 ± 0.01 respectively,with difference being significant between the group of VES pretreated OLTx and the group of OLTx ( P < 0.05 );When the mice were pretreated with VES,the markers of hepatocyte injury and UCP-2 levels were decreased.Conclusion VES pretreatment had some protective effects on OLTx.