肾移植患者胆固醇酯转运蛋白基因多态性与脂代谢的关系

Relationship between polymorphysm of the gene encoding cholesterol ester transfer protein and serum lipid level in renal transplant patients

目的 探讨肾移植患者胆固醇酯转运蛋白 (CETP)基因多态性与脂代谢的关系。方法 生物化学方法检测肾移植患者移植前及移植后 6～ 8个月时的血脂水平 ;多聚酶链反应 -限制性片断长度 (PCR RFLP)方法检测CETP内含子 1TaqⅠB与内含子 8MSPⅠ位点基因多态性。 结果 肾移植患者移植前甘油三脂 (TG)水平显著高于正常对照组 ,血清总胆固醇 (TC)、高密度脂蛋白胆固醇(HDLC)、低密度脂蛋白胆固醇 (LDLC)、脂蛋白 (a) [Lp(a) ]水平均显著低于正常对照组 ;移植后TC、TG、HDLC、LDLC、载脂蛋白 (ApoB、ApoE)水平均显著高于对照组及移植前水平。肾移植患者CETPTaqⅠB与MSPⅠ位点基因型及等位基因频率的发生率与正常对照组差异无显著性。CETPTaqⅠB基因型为B1/B1的肾移植患者 ,移植前TG、ApoB水平显著增高 ,HDLC水平显著降低 ,移植后表现为TG水平显著增高。肾移植患者CETPMSPⅠ位点不同的基因型间血脂水平差异无显著性。结论 肾移植患者易发生高脂血症。CETP内含子TaqⅠB位点基因多态性可显著性影响肾移植患者TG水平 ,基因型为B1/B1的患者易发生高甘油三酯血症。

Objective To investigate the effect of the gene polymorphism of cholesterol ester transfer protein (CETP) on the serum lipid levels in renal transplant patients.Methods Serum total cholesterol (TC),triglyceride (TG),low density lipoprotein (LDL),high density lipoprotein cholesterol (HDLC),apolipoproteins (Apo A1,B,E) and lipoprotein(a) [Lp(a)] were measured. Polymerase chain reaction-restriction fragment length polymophism (PCR-RFLP) was used to detect CETP gene polymorphism in renal transplant recipients.Results Serum levels of TC,TG,HDLC,LDLC,ApoB,ApoE in the renal transplant recipients were increased significantly after transplantation. The allele frequency and the distribution of the TaqⅠ(intron 1) and MSPⅠ(intron 8) genotypes showed no significant difference between the controls and the renal transplant recipients. The serum TG level was significantly higher in renal transplant recipients with the genotype TaqⅠB1/B1 than those in the patients with genotype TaqⅠ B1/B2 and B2/B2. But there was no statistical difference among the serum lipid levels in renal transplant recipients with different MSPⅠgenotypes.Conclusion The serum lipid levels were increased significantly in transplant after transplantation,and the patients with CETP genotype TaqⅠB1/B1 liable to develop hypertrglyceridemia.