马鞍山、苏州地区5岁以下腹泻儿童轮状病毒G、P分型研究

Clinical study on rotavirus serotype G and P patterns among children under 5 years old with acute diarrhea in Maanshan and Suzhou city

目的研究马鞍山和苏州地区5岁以下腹泻儿童轮状病毒G型和P型的分型。方法对马鞍山和苏州地区5岁以下急性腹泻儿童粪便标本1267份,采用酶联免疫吸附试验和巢式-聚合酶链式反应检测轮状病毒感染和血清型。结果轮状病毒阳性标本378份,主要来自2岁以下婴幼儿;血清型G型是优势株共250株,其中G1100株,G28株,G3111株,G43株,G93株,混合感染4株,未能分型21株(8.40%;马鞍山以G1型为主(58.54%苏州以G3型为优势株(47.85%;P基因型两地区流行株为P4和P8,分别占52.33%和47.67%,G1P4成为仅次于G1P8的GP组合。结论在苏州和马鞍山地区5岁以下儿童轮状病毒腹泻中,流行的血清型分别为G3和G1,P基因型流行株为P4和P8。

Objective To investigate the distribution of G and P serotypes of rotavirus among children under 5 years old with acute diarrhea in Suzhou and Maanshan city. Methods Total of 1267 stool specimen were collected from children under 5 year old with acute diarrhea.Enzyme-linked immunosorbant assay(ELISA)and nested polymerase chain reaction(Nested-PCR) was used to detect rotavirus and serotypes.Results Of 1267 stool specimens,378 were detected to be positive.Most of the positive specimens were from those under 2 years old.Serotype G was the prevalent type in the two cities with total number of 250 traits.Among them,the number of G1 was 100;G3 was 111,while the G2,G4,G9 and mixed infections were 8,3,3,4 in number and the unidentified type were 8.40%(21/250).The prevalent genotype were P and P in Maanshan and Suzhou,accounting for 52.33% and 47.67%,respectively.G1 was the most prevalent type in Maanshan, accounting for 58.54% and G3 was the most prevalent type in Suzhou (47.85%),respectively. Conclusions The most prevalent serotypes of rotavirus were G3 and G1 in Suzhou and Maanshan,respectively for children under 5 years old with acute diarrhea. The most prevalent genotypes were P and P .