“心俞”-“厥阴俞”穴位贴敷对实验性动脉粥样硬化及高血脂症疗效的观察

Effects of Acupoint-application of “Qiang Xin Tie" on Vascular Structure, Serum High Blood Lipid and High Cholesterol Contents in Artherolerosis Rabbits

目的 :观察“心俞” “厥阴俞”贴敷强心卡帖对实验性动脉粥样硬化家兔血管结构、高血脂和高胆固醇血症的影响。方法 :大耳白家兔共 48只 ,随机分为正常对照组 (n =6) ,模型组 (n =13 ) ,2 .5月实验组 (n =19)和 3 .5月实验组 (n =10 )。动脉粥样硬化模型用高脂、高胆固醇饲料喂养 2～ 3个月造成。测量股动脉平均血压 (MAP) ,显微镜下测量主动脉、腹主动脉及心肌血管中外膜及内膜的厚度 ;观察心脏大、中小冠脉血管分支堵塞情况 ;生化用酶法测定血清甘油三脂 (TG)、总胆固醇 (TCh)、高密度脂蛋白胆固醇 (HDL C)、低密度脂蛋白胆固醇 (LDL C)的含量。喂养上述特化饲料前两周 ,开始将强心帖贴敷于“心俞” “厥阴俞”、心前区 ,共贴敷 2 .5～ 3 .5个月。结果 :①对照组、模型组、实验组 (3 .5月 )MAP值相比组间虽无显著差异 ,但实验组的值低于模型组的趋势还是比较明确的 ;②贴敷强心帖 2 .5个月时 ,实验组主动脉、腹主动脉内膜的厚度低于模型组 ;实验组血清LDL C含量略低于模型组 ;③贴敷 3 .5个月时 ,实验组腹主动脉和心肌冠脉血管内膜均低于模型组 ,对照组心肌冠脉血管分支的结构绝大多数为正常 ,模型组半数或半数以上发生血管严重堵塞 ,而实验组半数以上为轻度堵塞 ;④贴敷 3 .5个月时 ,实验组血清TG、

Objective: To observe the effect of application of “Qiang Xin Tie' (Plaster for Strengthening Cardiac Performance) at “Xinshu'(BL 15) and “Jueyinshu'(BL 14) on vascular structure, serum high blood lipid and high cholesterol levels in artherolerosis rabbits. Methods: 48 healthy rabbits were randomly divided into control group (n=6), model group (n=13), 2.5 month (2.5 M, n=19) and 3.5 month (3.5 M, n=10) groups. Artherolerosis model was established by feeding the animal with specialized forage (12% yolk powder, 7% pig fat and 0.7% cholesterol and common forage) for 2 and 3 months respectively. Mean arterial pressure (MAP) of the femoral artery, the thickness of the endangium, tunicae media plus externa of the aorta, abdominal aorta and the endangium of the coronary vessels, the blockage state of the coronary vessels were observed and detected under microscope (micrometer). Serum triglyceride (TG), total cholesterol (TCh), high density lipoprotein cholesterol (HDL C) and low density lipoprotein cholesterol (LDL C) contents were assayed by zymochemical techniques. Two weeks before feeding the animals with the specialized forage, “Qiang Xin Tie' was applied to bilateral “Xinshu'(BL 15), “Jueyinshu'(BL 14) and the precordial region and replaced by a new one once every day or every other day, continuously for 2.5～3.5 months. Results: ① Comparison among control, model and 3.5 M groups showed that MAP of 3.5 M group was lower than that of model group (148.00±23 mmHg vs 159.71±14.07) and was close to that (145.00±17.61 mmHg) of control group. ② In 2.5 M group, the intimal thickness values of both aorta and the abdominal aorta and serum LDL C were lower than those of model group in spite of no statistical difference. ③ In 3.5 M group, the intimal thickness values of the abdominal aorta and the coronary vessels (larger, middle and smaller branches) were lower than those of model group; majority of the coronary vessels (94.4%) of control group were normal in the structure, 50.0% ～71.4% vessels had severe block, while 44.4%～61.1% in 3.5 M group had light block in the intravascular lumen. ④ In 3.5 M group, serum TG, TCh and LDL C were significantly lower than those of model group (P<0.05～0.01) and 2.5 M group (P<0.05～0.001). No significant changes were found in serum HDL C level. Conclusion: Acupoint application of “Qiang Xin Tie' may check the developing process of artherolerosis to a certain degree, lower blood pressure and blood lipid in the experimental artherolerosis rabbits.