电针及去卵巢术对大鼠脑内催乳素释放肽的影响

Influence of Electroacupuncture on Intra-cerebral Prolactin Releasing Peptide Level in the Ovariectomized Rats

目的 :通过观察去卵巢术和电针对大鼠脑内催乳素释放肽 (prolactinreleasingpeptide,PrRP)的影响 ,以进一步探讨PrRP是否参与生殖内分泌功能调节及其在电针调整雌性生殖内分泌功能异常中的作用。方法 :动物分为假手术组 (Sham)、去卵巢组 (OVX)和去卵巢 +电针组 (OVX+EA)。用放射免疫法 (RIA)测定电针前后去卵巢大鼠血清中催乳素 (prolactin ,PRL)含量的变化 ;免疫组化和RT PCR观察电针及去卵巢对大鼠脑内PrRP蛋白及mRNA表达的影响。结果 :OVX大鼠与Sham组相比 ,血清PRL水平、孤束核PrRP免疫阳性细胞数、终纹床核PrRP阳性纤维相对光密度值及延髓PrRPmRNA都显著降低 (P <0 .0 1或 0 .0 5) ;OVX +EA大鼠与OVX组相比 ,以上指标均显著升高 (P <0 .0 5)。结论 :PrRP可能参与雌性大鼠下丘脑 垂体 卵巢轴 (HPOA)的调节 ;电针对HPOA异常功能的调整作用 。

Objective: To investigate the effect of electroacupuncture (EA) and ovariectomy on the expression of prolactin releasing peptide (PrRP) in the brain of rats and to probe whether PrRP is involved in the regulation of reproductive endocrine system and to study the underlying mechanism of EA in normalizing the function of reproductive endocrine system. Methods: 24 SD rats were randomly and evenly divided into sham operation group, ovariectomized (OVX) group and OVX+EA group. EA (2～3 Hz, 1～2 mA) was applied to “Guanyuan” (CV 4), “Zhongji” (CV 3), bilateral “Zigong” (EX CA 1) and unilateral “Sanyinjiao” (SP 6) for 30 min, once daily and continuously for 3 days. Serum prolactin contents before and after EA were assayed by radioimmunoassay technique. Expression of PrRP protein and mRNA was analyzed by using immunohistochemical and reverse transcription polymerase chain reaction (RT PCR) methods. Results: Serum prolactin content, the number of PrRP immunoreaction (IR) positive neurons in nucleus tractus solitarii (NTS) of medulla oblongata and the relative optical density value of PrRP positive fibers in bed nucleus of stria terminalis (BST) and expression level of PrRP mRNA in medulla oblongata in OVX group were significantly lower than those of sham operation group (P<0.05～0.01), while those of EA+OVX group were significantly higher than those of OVX group (P<0.05). Conclusion: PrRP may be involved in the regulation of female hypothalamus pituitary ovary axis (HPOA) and EA may normalize the deviant function of HPOA via affecting PrRP expression in medulla oblongata.