益气温阳护卫汤对缓解期哮喘患者气道反应性的影响及作用机理研究

Study on Effects of Yiqiwenyanghuweitang on Airway Responsiveness of Asthma at Remission Stage and Mechanism of the Formula

目的 :探讨益气温阳护卫汤对缓解期哮喘患者气道反应性的影响及作用机理。方法 :将 6 2例支气管哮喘缓解期患者随机分为益气温阳护卫汤组 (治疗组 )和普米克气雾剂组 (对照组 )两组 ,每组各 31例。治疗组服用益气温阳护卫汤治疗 ,对照组使用普米克气雾剂吸入治疗 ,两组连续用药 6个月。两组治疗前后检测PC2 0 FEV1作为气道反应性指标。并进行痰液EOS计数 ;采用双抗体夹心ELISA法测定痰液IFN γ、IL 5 ;采用瑞典PharmaciaCAPSystem荧光酶标法测定ECP。结果 :治疗后两组PC2 0 FEV1值与治疗前比较均有显著性差异 (P <0 0 1 ) ;但两组治疗后比较无统计学意义 (P >0 0 5 )。治疗后两组患者痰液IFN r、IL 5、EOS、ECP与治疗前比较有显著性差异 (P <0 0 5或P <0 0 1 ) ;且治疗后两组患者痰液IL 5、EOS、ECP比较无显著性差异 (P >0 0 5 ) ,但痰液IFN γ治疗组明显高于对照组(P <0 0 5 )。结论 :益气温阳护卫汤具有明显降低哮喘患者气道反应性的作用 ,其可能通过减轻气道炎症实现。

Objective: To study on the effect of Yiqiwenyanghuweitang on airway responsiveness of the asthma patients at remission stage and the mechanisms of the formula. Methods: Sixty one patients with asthma at remission stage were randomly divided into two groups. Control group (31 cases) was treated with Pulmicort Aerosol and treatment group (31 cases) with Yiqiwenyanghuweitang continuously for six months. PC20 FEV1 used as the index of airway responsiveness in the two groups was measured before and after treatment respectively. The count of eosinophil (EOS) in the induced sputum was measured; IFN γ and IL 5 in the sputum were measured by double antibody sandwich ELISA method; And eosinophil cationic protein (ECP) was measured by fluorescence enzyme labeled method with Sweden Pharmacia CAP system. Results: The values of PC20 FEV1 of the two groups after treatment have significant difference compared with that before treatment ( P < 0.01); but there was no statistical significance between two groups after treatment ( P >0.05). Compared with the indexes before treatment, the contents of IFN γ, IL 5, EOS and ECP had marked difference in the two groups after treatment ( P <0.05 or P <0.01); And the contents of IL 5, EOS, ECP in sputum in the two groups after treatment has no significant difference compare to each other ( P >0.05); But the level of IFN γof the treatment group was significantly higher than that of the control group ( P <0.05). Conclusions: Yiqiwenyanghuweitang could markedly decrease the airway responsiveness of the patients with asthma by alleviating their airway inflammation.