他莫昔芬对结肠癌细胞株生长的抑制作用

Inhibitory effect of tamoxifen on colon cancer cell lines

目的 研究他莫昔芬 (tamoxifen ,TMX)对结肠癌细胞株生长的抑制作用。方法 选用Caco 2和LoVo两种结肠癌细胞株 ,采用酸性磷酸酶法测定 1× 10 -4～ 1× 10 -9mol/LTMX并联合应用 5 氟尿嘧啶 (5 FU)时对细胞生长的抑制作用。以流式细胞仪测定细胞周期的改变及凋亡。结果 TMX对两种细胞株均有一定的抑制作用 ,且TMX对细胞生长抑制作用呈剂量依赖性。TMX +5 FU有协同作用 ,其作用较单药强。流式细胞仪检测表明与对照组相比 ,TMX组、5 FU组及联合用药组对细胞株均有不同程度的生长抑制作用。尤其是联合用药组 ,TMX可加强 5 FU的作用 ,强烈地诱导细胞凋亡 ,凋亡细胞比例为 33.2 6 % (LoVo细胞株 )和 4 5 .87% (Caco 2细胞株 )。结论 TMX可抑制结肠癌细胞株生长 ,并可加强 5 FU的作用。其机制可能是通过雌激素受体 (ER)

Objective To evaluate the effect of tamoxifen (TMX) on growth and apoptosis of colon cancer cell lines. Methods Colon cancer cell lines, LoVo and Caco-2 were treated with TMX ranged from 10 -4 to 10 -9mol/L and with combination of 5-FU. The growth of the cells was detected by using acid phosphatase test. Apoptosis of the cells was measured by flow cytometery and the change of cell-cycle phases. Results TMX showed dose-dependent growth inhibition on both cell lines. Combination of TMX and 5-FU showed significant growth inhibition in comparing to either agent used singly. It could induce apoptosis of the colon cancer cell lines with an increase in percentage of apoptotic cells up to 33.26% and 45.87%, respectively. Conclusion TMX inhibits colon cancer cell growth. The effect of 5-FU on colon cancer cells can be enhanced by TMX .Induction of apoptosis through estrogen receptor β might be one of the mechanisms of TMX inhibiting colon cancer cell growth.