摘要
比较了离子交换法得到的固定化小麦酯酶和固定化鸡肝酯酶的主要性质与相应的游离酶的性质。结果表明 ,固定化小麦酯酶的最适pH在 5 5附近 ,比游离小麦酶的最适 pH增加了0 8个 pH单位 ;固定化鸡肝酯酶的最适pH在 8 0附近 ,比游离鸡肝酯酶的最适 pH增加了 0 5个pH单位 ;固定化小麦酯酶的最适温度与游离酶的相当 ,但酶活力 温度曲线的峰形变小 ;固定化鸡肝酯酶的最适温度比游离鸡肝酯酶的下降了 5℃ ,酶活力 温度曲线的峰形也略变陡峭。这 2种固定化酯酶对有机磷类农药 (敌敌畏 )
Esterases from wheat and chicken liver were immobilized by ion exchanging method The properties of immobilized wheat and chicken liver esterase have been compared with their corresponding free enzymes The results showed that the optimum pH values of both immobilized wheat esterase (IWE) and chicken liver esterase (ICLE) were 0 8 and 0 5 pH unit higher than those of their free esterases respectively, the optimum temperature of IWE was similar to that of free wheat esterase while the optimum temperature of ICLE reduced 5℃ from that of its corresponding free enzyme Both IWE and ICLE became more sensitive to the inhibition of organophosphate pesticide (dichlorvos) than their free enzymes
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2004年第7期38-42,共5页
Food and Fermentation Industries
基金
国家自然科学基金会项目 (No.5 0 1 76 0 32 )
上海市科委"启明星"计划资助项目 (0 2QF1 4 0 30 )
上海市教委青年基金项目(0 2GQ2 1 )资助
