醋酸铅对雄性小鼠生殖功能的影响

Study of effects of lead acetate on reproductive function in male mice

给雄性小鼠灌胃染毒醋酸铅 10mg/kg、 2 0mg/kg和 40mg/kg ,每天 1次连续 3 5d。应用分光光度法测定血清和睾丸中酶活性 ,按标准方法测定小鼠生育力 ,并对睾丸、附睾进行组织病理学检查。结果表明 ,雄鼠受精率、雌鼠妊娠率、平均每窝活胎率及睾丸匀浆中的 β G和LDHx随染毒剂量增加而降低 ,死胎率和吸收胎率随染毒剂量增加而升高 ,与阴性对照组比较差异均有显著性 (P <0 0 5 ) ,并呈现明确的剂量 反应关系 ,r值依次为 -0 412、 -0 42 0、-0 5 3 7、 -0 5 3 8、 -0 617、 0 5 42和 0 5 3 5。雄鼠交配率 ,血清和睾丸匀浆中G 6 PD、ACP、LDH仅在 40mg/kg组与阴性对照组比较差异有高度显著性和显著性 (P <0 0 1或P <0 0 5 )。说明染毒醋酸铅≥ 2 0mg/kg剂量可损害雄性小鼠生殖功能。

Male mice were orally administered with lead acetate of10mg/kg,20mg/kg and40mg/kg,respectively,via gastric tube once daily for35days,consecutively.Activities of glucose-6-phosphate dehydrogenase(G-6-PD),acid phosphatase(ACP),β-glycosidase(β-G),lactic acid dehydrogenase(LDH)and lactic acid dehydrogenase isoenzyme(LDHx)in the serum and testis ho-mogenate of male mice were determined with spectrophotometry and their fertility was measured with a standard method.Their testis and epididymis were examined histopathologically.Results showed that insemination rate in male mice,pregnancy rate in female mice,av-erage number of live birth of mice per nest and activities ofβ-G and LDHx in testis homogenate decreased with the increase of dose of lead acetate,as compared to the control mice,while stillbirth rate and embryo resorption rate increased with dose of lead acetate,in a significant dose-response pattern,as compared with the negative control group(P<0.05),with coefficients of regression of-0.412,-0.420,-0.537,-0.538,-0.617,0.542and0.535,respectively.While,mating rate in male mice,activities of G-6-PD,ACP and LDH in the serum and testis homogenate in the rats exposed to40mg/kg of lead acetate were significantly different from those in the negative control group(P<0.01or P<0.05),indicating that fertility in male mice could be damaged by exposed to≥20mg/kg of lead acetate.