针刺对快速老化小鼠SAMP10氧化应激相关基因表达的影响

Influence of acupuncture on expression of relative oxidation stress gene in accelerated aged rat SAMP10

[目的]探讨快速老化小鼠SAMP10脑衰老的机制 ,揭示“益气调血、扶本培元”针法改善SAMP10脑衰老症状的部分机制。[方法]应用cDNA阵列技术 (cDNAArray) ,观察快速老化小鼠SAMP10与正常老化小鼠SAMR1前脑588个基因表达的差异 ,同时观察“益气调血、扶本培元”针法对上述基因表达的影响。[结果]SAMP10与SAMR1相比 ,谷胱甘肽S转移酶、热休克蛋白86、成纤维细胞生长因子8、NF -kappa -B转录因子p65亚单位4个与氧化应激反应有关的基因表达上调 ,而针刺可使这些基因的表达下调。[结论]SAMP10的快速老化可能与其脑内的氧化应激水平升高有关 ,针刺可改善氧化应激水平进而达到延缓脑衰老的作用。

Objective] To explore the partial mechanism of brain senescence in SAMP10,and the improvement function of“Yiqitiaoxue,fubenpeiyuan”on senile symptoms of brain.[Method] cDNA Array technique was used,and the expression difference of588genes of front brain was observed in SAMP10and SAMR1,meanwhile the influence of“Yiqitiaoxue,fubenpeiyuan”on the expression of the above mentioned gene was observed.[Results]Compared with SAMR1,the expresˉsion of glutathione transferase S,HSP86,fibroblast growth factor FGF,and NF-kappa-B p65subunit4gene relative to oxˉidation stress response in SAMP10was upregulated,while acupuncture could decrease the expression.[Conclusion] The rapid senescence of SAMP10may be related to the upregulated level of oxidation stress gene in the brain,acupuncture can improve it and so as to retard brain senescence.