非霍奇金淋巴瘤EB病毒感染和HLA-DQ抗原与p53蛋白表达关系研究

Significance of epstein-barr virus and the expression of HLA-DQ antigen and p53 protein in non-hodgkin’s lymphoma

目的 :探讨非霍奇金淋巴瘤 (non Hodgkin’slymphoma ,NHL)EB病毒感染与HLA DQ抗原表达和p5 3蛋白表达阳性率及其相关性。方法 :应用免疫组化SP法检测 160例不同病理类型的NHL石蜡包埋组织的LMP 1阳性率和HLA DQ抗原表达与p5 3蛋白表达率。 结果 :B细胞NHL (B NHL)、T细胞NHL(T NHL)的EB病毒检出率分别为 2 8 9% ( 3 9/13 5 )和 16 0 % ( 4 /2 5 )。HLA DQ抗原表达的阳性率 ,B NHL和T NHL分别为 2 5 2 % ( 4 /13 5 )和 2 8 0 %( 7/2 5 )。EB病毒感染程度与HLA DQ抗原表达阳性程度呈正相关。p5 3蛋白表达阳性率 ,B NHL和T NHL分别为 48 9% ( 66/13 5 )和 3 2 0 % ( 8/2 5 )。结论 :EB病毒潜伏感染在NHL发病中起一定作用。EB病毒、HLA DQ之间可能存在内在联系并相互影响 ,HLA DQ抗原的表达可能会增强EB病毒进入机体的易感性。而NHL中p5

OBJECTIVE:Epstein-Barr virus infection and the expression of HLA-DQ antigen and p53 protein in non-Hodgkin's lymphoma(NHL) and the correlations were studied.METHODS: LMP-1 and the expression of HLA-DQ antigen and p53 protein in 160 cases of the paraffin embodied tissues of NHL including different pathological types were detected using streptavidin-peroxidase (SP) immunohistochemistry.RESULTS: The detectable rates of EB virus in B-NHL and T-NHL were 28.9%(39/135) and 16.0%(4/25) respectively. The positive rate of HLA-DQ antigen expression in B-NHL and T-NHL were 25.2%(4/135) and 28.0%( 7/25) respectively. There was a positive correlation between the extent of EB virus infection and the grade of HLA-DQ expression. The positive rate of p53 protein expression in B-NHL and T-NHL were 48.9%(66/135) and 32.0%(8/25) respectively.CONCLUSIONS: The latent infection of EB virus may pay an important role in the pathogenesis of NHL. There was probably an inherent relation and influence each other between EB virus and HLA-DQ. The antigen expression of HLA-DQ may increase the susceptibility of EB virus entry. However, there was not a relation between EB virus and p53 in NHL.