抗CXCR4单克隆抗体对HL-60细胞粘附性及Bcl-2、Fas蛋白表达的影响

Effects of Anti-CXCR4 Monoclonal Antibody on Adhesiveness of Human Acute Myelocytic Leukemia Cell Line HL-60 and Expression of Bcl-2,Fas Proteins

本研究通过观察抗CXCR4单克隆抗体 (12G5 )对HL 6 0细胞粘附性及Bcl 2、Fas蛋白表达的影响 ,探讨趋化因子SDF 1在维持HL 6 0细胞生存中的作用。培养HL 6 0细胞 ,并再与骨髓基质细胞共培养 ,在抗CXCR4单克隆抗体阻断SDF 1生物活性后 ,观察骨髓基质层上HL 6 0细胞的粘附情况并测定其粘附率 ;应用免疫组织化学技术检测HL 6 0细胞Bcl 2及Fas蛋白的表达。结果显示 ,12G5显著降低HL 6 0细胞的粘附率 ,与此同时Bcl 2表达下调 ,Fas表达上调。结论 :抑制SDF 1活性可一定程度地阻抑白血病细胞生存 ,促进凋亡。

To study the importance of chemokine SDF-1 in surviving of acute myelocytic leukemia cells HL-60,the adhesion ability of HL-60 and expression of Bcl-2,Fas protein when SDF-1 activity was blocked by anti-CXCR4 monoclonal antibody (12G5) were compared with those detected before MAb incubation,in this experiment,HL-60 cell were cultured and co-cultured with normal marrow stromal cell. The adhesion rate was detected while the expression of Bcl-2 and Fas proteins were assayed by immunohistochemical technique when SDF-1 activity was inhibited. The results showed that cell adhension rate of HL-60 decreased while the expression Bcl-2 decreased and Fas increased. It is concluded that inhibition of SDF-1 activity increases cell apoptosis and thus reduces life-span of leukemia cell at certain level.