两种牙科涡轮手机停转次数对回吸作用影响的研究

Comparison of Preventive Effects of Two Kinds of Dental Handpieces on Viral Contamination at Different Rotating Times

目的 比较防回吸手机和普通手机在不同停转次数条件下 ,对病毒回吸进入手机内部的影响。方法 将两种手机分别浸泡在 10 - 6 μg μl的HBV质粒溶液中 ,模拟临床手机操作方式旋转 5次和 10次 ,每次 10s,用标准纸尖对驱动气管口、冷却水管口、冷却气管口、余气管口、水箱进行取样 ,用PCR方法进行检测。结果 相同手机在同一取样部位 ,不同停转次数的回吸发生率间无显著性差异 (P >0 0 5 ) ;而新型防回吸手机无论在停转 5次 ,还是停转 10次时 ,在冷却水管口和冷却气管口的回吸发生率均明显低于常规手机 ,其差异有显著性 (P <0 0 5 ) ;但在驱动气管口、余气管口、水箱 ,两种手机的回吸发生率间无显著性差异 (P >0 0 5 )。结论 新型防回吸手机能有效减少手机在停气瞬间的回吸效应 ,减轻了手机的污染。

Objective It has been demonstrated that when a high-speed handpiece stops rotating, negative pressure will form. Thus, contaminating fluid in which there are many kinds of bacteria and viruses from the external environment will retract into various compartments of the handpiece and the dental unit. The purpose of the study is to compare the preventing effect of antisuction designed handpiece and conventional handpiece on viral contamination at different rotating times.Methods Twenty handpieces with or without antisuction device (10 of each) were used in the study. Each handpiece was submerged into 10+{-}6 μg/μl HBV particle solution rotating 5 and 10 times respectively (every time rotating for 10 seconds). Samples were obtained from the water line and chip air line of the handpieces and examined by RT-PCR.Results At the same rotating times, there was statistical significance of the viral concentration between the two kinds of handpieces (P<0.05). However, there was no statistical significance of the viral concentration between different rotating times in each group (P>0.05). Conclusion Contamination taking place in both water and air lines of dental handpiece was not enhanced by increasing the number of rotating times of the handpiece. The antisuction devices installed into the water line and chip air line were demonstrated to prevent viral contamination effectively.