人乳牙牙根吸收过程中根面破骨细胞的观察

Observation of Osteoclasts on the Root Surface during Human Deciduous Teeth Resorption

目的 观察人乳牙根吸收面的破骨细胞形态特点。方法 收集和固定人滞留乳牙 ,制备硬组织切片 ,经苏木精 -伊红、抗酒石酸酸性磷酸酶染色 ,在光镜和扫描电镜下观察破骨细胞。结果 在人乳牙根吸收面有多个破骨细胞 ,呈重叠状态 ,细胞形态不规则 ,体积大 ,细胞周缘可见丝状突起 ,多核 ,被吸收的牙本质表面高低不平。结论 人滞留乳牙牙根吸收面存在大量的破骨细胞 ,是研究人破骨细胞的一个来源。

Objective To observe osteoclasts on the resorbing surface of human deciduous teeth.Methods After fixing the collected deciduous teeth, we prepared the tooth slices without decalcification, treated them with HE and TRAP dyestuff, and observed the osteoclasts under light and scanning electron microscope.Results There were large quantity of various forms of overlapping and huge osteoclasts with many nuclei and silk-like protuberances on the resorbing surface of deciduous teeth. The multinucleated osteoclasts align on the surface of coarse dentin.Conclusion On the resorbing surface of human deciduous teeth there are large amount of osteoclasts which can be used as a source of studying human osteoclast.