口腔鳞状细胞癌Fas基因表达与细胞凋亡关系的研究

Study on the Relationship between Fas Expression and Apoptosis in Oral Squamous Cell Carcinoma

目的 探讨Fas基因mRNA及蛋白表达水平与人口腔鳞状细胞癌细胞凋亡的关系。方法 应用Northern杂交、双参数流式术 (TUNEL法 )检测人口腔鳞状细胞癌中FasmRNA及蛋白的表达 ,鳞癌细胞的细胞周期与凋亡水平。结果 5例正常口腔黏膜标本中均有FasmRNA及蛋白的表达。在口腔鳞癌组织中 ,FasmRNA及蛋白的表达与口腔鳞癌组织病理学分级有关 (P <0 0 0 5 ) ,与口腔鳞癌组织分化程度和凋亡指数呈正相关。结论 口腔鳞癌中Fas基因的表达与组织病理学分级及细胞凋亡之间关系密切。

Objective To investigate the relationship of Fas mRNA and protein expression and apoptosis in human oral squamous cell carcinoma.Methods Northern blot and flow cytometry (TUNEL method) were used to detect the expression of Fas mRNA and Fas protein, cell cycle and apoptotic level in oral squamous cell carcinoma. The relationship between Fas gene expression and OSCC apoptosis was analyzed statistically.Results Fas mRNA and protein could be detected in all five normal oral mucosa specimens. There was positive correlation between expression of Fas mRNA/protein and cell differentiation as well as apoptosis in OSCC(P<0.005).Conclusion The expression of Fas gene was highly correlated with the differentiation and apoptosis in OSCC.