重症肌无力患者血清白细胞介素-10水平变化的研究

Investigation of the variation of serum interlenkin-10 levels in myasthenia gravis patients

目的 :探讨重症肌无力 (MG)患者血清白细胞介素 (IL) 10的变化及其临床意义。方法 :采用双抗体夹心ELISA法检测 36例未经免疫抑制治疗和 30例已使用糖皮质激素 (GC)系统治疗的MG患者及 2 0例健康献血者的血清IL 10水平。结果 :血清IL 10水平在MG患者高于健康者 (P <0 0 1) ,经GC治疗后 ,其水平明显降低 (P <0 0 5 ) ,但仍高于健康者 (P <0 0 5 ) ;在AchRab阳性患者高于AchRab阴性患者 (P <0 0 5 )和正常对照者 (P <0 0 1) ,且与AchRab滴度呈正相关 (P <0 0 1)。IL 10分别在MG未治组和GC治疗组中 ,全身型均高于眼肌型 (P <0 0 5 ) ;病情重者均高于病情轻者 (P <0 0 1) ;急性期均高于慢性期 (P <0 0 5 ) ;伴胸腺异常者均高于胸腺正常者 (P <0 0 5 )。结论 :MG患者血清IL 10水平显著增高 ;IL 10与MG临床特点和AchRab的产生有关 。

Objective:To investigate the variation of serum interleukin(IL)-10 in myasthenia gravis (MG) patients and their clinical significance. Methods:The serum IL-10 are measured in 36 cases of pretreated and 30 cases post-treated with glucocorticosteroid MG patients and 20 normal persons. Results:The serum IL-10 levels are much higher in patients with MG than that in controls (P<0.01),after treated with glucocorticosteroid, it's level decreased much(P<0.05), but higher than that in controls(P<0.05). The serum IL-10 levels are higher in MG patents whose serum AchRab are positive than that in those serum AchRab are negative(P<0.05) and than that in controls (P<0.01). There are positive relevance between IL-10 and AchRab in MG patients(P<0.01). The serum IL-10 levels are higher in MG patients of generalized type than that in those of ocular type(P<0.05),are higher in severe patients than that in mild patients(P<0.01),are higher in the acute period than that in the chronic period(P<0.05),are higher in patients who accompany with thymom or thymic hyperplasia than that whose thymus are normal(P<0.05).Conclusion:There are significant increase of IL-10 in MG patients. The serum IL-10 levels are related to the clinical feature and the production of AchRab, which play an important role in the immunopathological process of MG.