HPLC法测定复方降压平片中5个成分的含量

Determination of five components in compound hypotensive tablet by HPLC

目的 建立复方降压平片中 5个成分 (利血平、氢氯噻嗪、氨苯蝶啶、氯氮和硫酸双肼屈嗪 )含量的高效液相色谱测定方法。方法 采用氰基键合硅胶色谱柱 ,分别以乙腈 0 0 1mol·L- 1 庚烷磺酸钠溶液 (pH 2 7) (7∶3)和 (5∶5 )为流动相 ;利血平、氢氯噻嗪、氯氮的检测波长为 2 6 7nm ,氨苯蝶啶、硫酸双肼屈嗪的检测波长分别为 36 0nm和310nm。结果 所有实验都经过了线性关系、回收率、样品稳定性等试验研究 ,均满足含量测定方法学的要求。

Aim To establish a method for the determination of the five components (reserpine, chlordiazepoxide, hydrochlorothiazide, dihydralazine sulfate, triamterene) in compound hypotensive tablet. Methods The chromatography was performed using a CN column with acetontrile-0.1 mol L -1 sodium heptasulfonate solution (7∶3) and (5∶5) as the mobile phases. The detection wavelength was 267 nm for reserpine, chlordiazepoxide and hydrochlorothiazide, 310 nm for dihydralazine sulfate, 360 nm for triamterene. Results The linear range of each component was tested, and the recovery and stability of each component was satisfactory, three lots of samples were determined using the method. Conclusion This is an accurate and credible quality control method for compound hypotensive tablet.