摘要
目的 应用源后衰变 (PSD)技术结合数据库检索鉴定二维蛋白质电泳 (2DE)胶上的蛋白质斑点。方法用已知多肽 (ACTH) 1839肽段和TPA被胰酶降解后的 1个肽段建立了PSD MALDI TOF MS方法。结果 应用已建立的PSD MALDI TOF MS法分别将 2DE分离后胶上的 2个未知蛋白质斑点鉴定为 4 0S核糖体蛋白S12和dnaK抑制蛋白。
Aim To identify protein spots on two dimentional protein electrophoresis (2DE) by post-source decay (PSD) technique associated with library search. Methods The PSD-MALDI-TOF-MS method was set up by a segment of ACTH and a peptide digested by trypsin for TPA. Results Two unknown protein spots on 2DE were identified as 40S ribosomal protein S12 and dnaK suppressor protein separately by established PSD-MALDI-TOF-MS method. Conclusion PSD technique has greater application prospects in peoteomics.
出处
《药学学报》
CAS
CSCD
北大核心
2004年第8期627-630,共4页
Acta Pharmaceutica Sinica
基金
国家高技术"863"计划资助项目 ( 2 0 0 1AA2 3 3 0 41)
国家自然科学基金资助项目 ( 3 0 0 70 3 77)
