香烟减毒添加剂对吸烟小鼠免疫功能及气管和肺结构的影响

Influence of cigarette additive for decreasing toxicity function in smoking mice

目的 :探讨香烟减毒添加剂对吸烟小鼠免疫功能及气管和肺结构的影响。 方法:昆明种小白鼠 15 0只 ,随机分为正常对照组、低剂量普通烟组、低剂量实验烟组、高剂量普通烟组、高剂量实验烟组 ,采用巨噬细胞吞噬试验检测巨噬细胞吞噬活性 ;通过淋巴细胞转化试验检测细胞免疫功能 ;通过测定白细胞数及胸腺、脾指数来检测小鼠非特异性免疫功能 ;常规方法制作组织切片 ,光镜观察小鼠气管和肺结构。 结果 :(1)普通烟组与正常对照组相比 ,小鼠的巨噬细胞吞噬率、白细胞总数明显下降 (P均 <0 .0 1) ;(2 )含减毒添加剂的实验烟组的小鼠巨噬细胞吞噬率、吞噬指数及白细胞总数均明显高于不含添加剂的普通烟组 (P <0 .0 1) ;(3)实验烟组的胸腺指数及脾指数略高于普通烟组 ,但差异无统计学意义。实验烟组的淋巴细胞转化率与普通烟组相比差异也无统计学意义 ;(4 )实验烟组及普通烟组小鼠的气管和肺内支气管均有炎症改变 ,实验烟组小鼠肺组织瘀血程度稍轻。 结论 :香烟减毒添加剂可以增强吸烟小鼠的免疫功能 ,但对气管、肺内支气管的损害无明显减轻作用。

Objective: To explore the effects of cigarette additive for decreasing toxicity on immune functions and the structures of trachea and lungs in smoking mice. Methods: Macrophage phagocytosis test for detecting the phagocytic activation,lymphocyte transformation test for detecting the functions of cellular immunity,by calculating the number of leukocytes and weighing the thymuses and spleens for detecting the non-special immune function,and the routine method to make tissue slices for observing the structure of trachea and lungs were observed. Results: (1) The phagocytic rate and phagocytic index of macrophages and leukocyte numbers were significantly lower in common smoking group than that in control group ( P <0.01),It indicated that the immune function of mice can be decreased by smoking. (2) The phagocytic rate and phagocytic index of macrophages and leukoctye numbers increased significantly more in experimental smoking group than that in common smoking group ( P <0.01). (3) The thymus index and spleen index were a little bit higher in experimental smoking group than that in common smoking group,but no difference on statistic. The lymphocyte transformation rate in experimental smoking group was similar with that in common smoking group. (4) There were inflammation in both experimental and common smoking group,without significant difference. Conclusion: The immune function of smoking mice can be increased by cigarette additive for decreasing toxicity,but the damages of trachea and lungs can not be reduced.