影响细胞周期逆转胆囊癌化疗耐受的研究

REGULATION ON GALLBLADDER CANCER CELL CYCLE REVERT ITS CHEMORESISTANCE ON DOXORUBICIN IN VITRO & IN VIVO

目的 通过使用生长抑素改变细胞周期状态 ,观察离体和在体状态下 ,人胆囊癌细胞株和裸鼠移植瘤对细胞增殖期特异性化疗药物阿霉素 (DOX)的敏感性的变化。方法 离体培养人胆囊癌细胞株 GBC- SD,MTT法观察生长抑素影响细胞周期后胆囊癌细胞对 DOX敏感性的变化 ;再构建胆囊癌裸鼠移植瘤 ,采用腹腔注射方法给药 ,分别观察生长抑素和阿霉素在单独以及联合用药情况下 ,胆囊癌移植瘤生长情况的变化。结果 生长抑素诱导胆囊癌细胞生长停滞于细胞周期的 S期 ,能明显降低癌细胞对 DOX的耐药性 (P<0 .0 5 ) ;胆囊癌裸鼠移植瘤对单独作用阿霉素有耐受性。联合用药能明显抑制移植瘤的生长 (P<0 .0 5 ) ;生长抑素在在体状态下的化疗增敏作用呈现浓度 -效应依赖现象。

Objective To investigate chemosensitivity of Doxorubicin(DOX) on human gallbladder cancer cell line GVC SD and its xenograft,after modulation of the gallbladder cancer cell cycle by Somatostatin.Methods Chemosensitivity of DOX on GBC SD in vitro was investigated using MTT assay after somatostatin pre treated.Then,xenograft of GBC SD cell line was found to investigate the effect of somatostain or DOX use only and combined use(Co use)on gallbladder cancer cell in vivo.Result Cancer cell growth arrest in S phrase reducing by somatostatin was detected,which resulted in reverting its chemotherapy resistance to DOX (P<0.05).Inhibition of GBC SD xenograft growth was not found by somatostatin or DOX use only.While Co use caused significant inhibition of GBC SD xenograft growth(P<0.05).And this inhibition was somatostatin concentration dependent.Conclusion Modulation on gallbladder cancer cell cycle could increase its chemosensitivity in vitro and in vivo.