摘要
采用1.5％粗纤维素酶加1.5％纤维素酶的混合酶,自Arthrobotrys guizhouense36-15,A. oligospora GA001, A.sp. Z307, A.dactyloides Z279,Monacrosporium eudermata Z326等5种捕食线虫真菌中获得了大量原生质体。比较了不同酶系统、渗透稳定液、菌丝生长培养基、菌龄、酶解温度等因素对Arthrobotrys guizhouense 36-15原生质体形成的影响。有机物渗透稳定液较无机渗透稳定液适合于从A.guizhouense 36-15菌株释放原生质体。0.6M葡萄糖稳定液配制的察氏培养基上,A.guizhouense 36-15菌株原生质体再生率可达48.9％。对原生质体释放和再生进行了详细的形态发生学观察和描述。
Protoplasts were abtaincd from Arthrobotry guizhouenes 36-15, A. oligospora GA001, A. sp. Z307, A. dactyloides Z279 and Monocrasporium eudermata Z326, with 1.5% crude cellulase and 1.5% cellulase R-10.The factors, such as, enzyme systems, stabilizers, media for mycclial growth, age of mycelium, temperatures and pH were studied upon influencing the protoplast formation in A. guinhouezse 36-15. The organic stabilizers were better than inorganic stabilizers for protoplasts formation from A. guizhouense 36-15. When 0.6M Glucose was used as stabilizer, about 48.9% of protoplast regenerated on Czapex-Dox agar media (MM). The morphological process of protoplast formation and regeneration was studied also.
关键词
线虫
真菌
原生质体
再生
nematode-trapping fungi
protoplast
regeneration
