鸡传染性支气管炎肾病变型的病原分离与鉴定

THE PATHOGEN ISOLATION AND IDENTIFICATfON OF AVIAN INFECTIOUS BRONKITIS (N APHROSIS)

通过鸡胚接种、传代,从病鸡肺、肾材料中分离出一株病毒。传至第五代,多数鸡胚表现发育停滞,胚体卷曲成球状,接种胚的致死率达到50％,部分鸡胚的肾脏尚可见尿酸盐沉积。结合病毒干扰试验、电镜观察和人工感染发病的结果,该病毒分离物确定为传染性支气管炎病毒。在临床上发病鸡群的剖检变化以肾脏和输尿管的病变为主,本次疫病确诊为鸡传染性支气管炎肾病变型。

One virus strain isolated by embryo inoculation and serial Passages with suspensions of tung and kidney collected from infected chicks.By the fifth generation most embryos were stunted and curted into a spherical form.The mortality of inoculated embryos reached 50% and some of them manifected nephrosis with accumulation of uric acid crystals. According to the results of virus interference test,electron microscope observation and artificial infection experiment,the virus isolate was identified as an infectious brinkitis virus.Clinically necropsy of affected chicks revealed swollen kidneys,with tubules and ureters often distended by uric acid crystals. This disease was definitely diagnosed as avian infectious bronkitis (nephrosis).