HPLC法测定通降胶囊中大黄素、大黄酚和大黄酸的含量

Determination of Emodin,Chrysophanol and Rhein in Tongjiang Capsules by HPLC

目的 :建立高效液相色谱法测定通降胶囊中大黄的大黄素、大黄酚和大黄酸含量。方法 :采用VARLAG公司C18柱(4.6mm× 2 5 0mm ,5 μm) ;流动相 :甲醇 - 0 .1%磷酸溶液 (85∶15 ) ,流速 1.2ml·min-1,检测波长 :4 4 0nm。结果 :大黄素在32～ 16 0ng、大黄酚在 80～ 4 0 0ng、大黄酸在 112～ 5 6 0ng范围内 ,进样量 (ng)与峰面积呈良好的线性关系。平均回收率分别为 99.2 % (大黄素 ,RSD =2 .96 % ) ,97.4 % (大黄酚 ,RSD =1.5 9% ) ,10 2 .9% (大黄酸 ,RSD =2 .4 0 % )。结论 :本法快速、简便、准确。

Objective: To establish a method for determination of emodin, chrysophanol and rhein in Tongjiang capsules by HPLC.Method: The column was Varlag C 18 , the mobile phase was melhanol 0.1% phosphate acid=(85∶15),the detecting wavelength was at 440 nm. Result: The emodin at 32 160 ng, the chrysophanol at 80 440 ng and the rhein at 112 560 ng, they all were well linear relationship. The average recoveries were 99.2% (emodin, RSD = 2.96% ), 97.4% (chrysophanol, RSD = 1.59% ), and 102.9% (rhrin, RSD = 2.40% ), respectively. Conclusion: The method is rapid,accurate and simple.