不同诱导条件对小鼠胚胎干细胞分化为心肌细胞的实验研究

Study of different methods of inducing embryonic stem cells in vitro to differentiate into cardiomyocytes

目的 :比较小鼠胚胎干细胞 (ESC)在不同诱导条件下分化为心肌细胞的分化比率。方法 :用鼠胚成纤维细胞(MEF)作为饲养层促进 ESC增殖并抑制其分化 ,用维甲酸 (RA )、二甲基亚砜 (DMSO)、转化生长因子β1 (TGF-β1 )、激活素 - A(activin- A)为分化诱导剂 ,采用三步法诱导 ESC分化为心肌样细胞 ,并比较各组分化比率。结果 :各组实验用的各种诱导剂均能诱导 ESC分化为心肌样细胞 ,尤以 TGF- β1 (2 ng/m l)、activin- A(2 0 ng/ml)及 2 0 %胎牛血清 (FCS)组成的分化培养基可以使其分化比率高达 88% ,显著高于其它各组 (P<0 .0 1)。结论 :以 MEF饲养层培养 ,TGF- β1 、activin- A作分化诱导剂可作为一种比较简便、稳定、高效的 ESC分化条件。

AIM:To compare the different methods of inducing mouse embryonic stem cells(ESC) in vitro to differentiate into cardiomyocytes. METHODS:Mouse embryonic fibroblast(MEF) feeders were used to promote the growth and to inhibit the differentiation of ESC. During the inducing process,RA,DMOS,TGF-β 1 and activin-A were used as inducing reagents and constituted six kinds of differentiating media. Then a three-step method was used to induce the differentiation of ESC. RESULTS:ESC could be induced in vitro to differentiate into cardiomyocytes in all groups,and the group induced by using TGF-β 1 and activin-A acquired the highest differentiating ratio. CONCLUSION:TGF-β 1 and activin-A were valuable reagents in inducing ESC to differentiate into cardiomyocytes.