摘要
目的 :研究枳实的最佳提取工艺及其质量分析的方法。方法 :选用溶剂用量 (A)、提取时间 (B)与提取次数 (C) 3个因素 ,每个因素选取 3个水平进行正交试验 ,采用薄层扫描法对枳实药材及其提取物中的有效成分辛弗林进行含量测定。结果与结论 :最佳工艺为用 6倍量 95 %乙醇回流提取 3次 ,每次 1.5h ;枳实药材中辛弗林含量为0 .4 38% ,枳实提取物中辛弗林含量为 4 .0 6 0 %。
Aim: To study the best extracting process of citrus aurantium L and establish a method for its quality analysis. Methods: An orthogonal test method was used. The solvent dosage(A), extracting time(B), and extracting times(C) were chosen as the three factors of the experiment, and three levels of each factor were chosen for the experiment. The content of synephrine in citrus aurantium and extracted substance was detected by TLC scanning method. Results and Conclusion: The factor A had certain effect on the rate of creaming, while the factor B and C had conspicuous effect. The best extracting process is extracting three times by using six times of 95% ethanol for 1.5 h every time. The content of synephrine in citrus aurantium is 0.438% and the content of synephrine in extracted substance is 4.060%.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2004年第5期765-767,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省科技攻关基金资助项目 0 12 4170 70 5
关键词
正交试验
枳实
辛弗林
提取工艺
orthogonal test
citrus aurantium
synephrine
extracting process
