特发性血小板减少性紫癜患者自体移植再生脾T淋巴细胞亚群的变化

Changes of T-lymphocyte subsets in regenerated tissue from autosplenic graft of patients with idiopathic thrombocytopenic purpura

目的 :探讨特发性血小板减少性紫癜 (ITP)患者自体移植再生脾T淋巴细胞亚群变化及其对免疫功能的影响。方法 :对 12例ITP患者行脾切除自体移植术 ,术后 6个月活检再生脾组织 ,应用碱性磷酸酶 抗碱性磷酸酶(APAAP)免疫组化和图像分析系统测量移植前脾及再生脾组织各区域T淋巴细胞亚群的细胞数密度。结果 :与移植前脾组织比较 ,再生脾CD3 + 细胞在边缘带 (MZ)和动脉周围淋巴鞘 (PALS)均明显减少 (P <0 .0 5 ) ,在红髓 (RP)则显著增加 (P <0 .0 1) ;CD4+ 细胞在再生脾的生发中心和PALS内显著减少 (P <0 .0 1) ;CD8+ 细胞在再生脾PALS和RP内均明显增加 (P <0 .0 1)。结论 :再生脾组织T淋巴细胞亚群分布发生改变 ,对防止自身血小板抗体的产生有益。

Aim: To study the changes of T-lymphocyte subsets in regenerated tissue from autosplenic graft of patients with idiopathic thrombocytopenic purpura(ITP) and its effects on immue function. Methods: Autosplenic tissue was transplanted into the omental sac in 12 cases of ITP.In the 6th month after transplantation,biopsy specimen of the regenerated splenic tissue was collected. T-lymphocyte subsets of the splenic tissue was analyzed by immunohistochemical APAAP technique and image analysis. Results: In regenerated splenic tissue,CD 3 positive cells remarkably decreased in marginal zone and periarterial lymphatic sheath (PALS)( P<0.05),and significantly increased in RP(P<0.01).CD 4 positive cells significantly decreased in germinal centers and PALS(P<0.01).CD 8 positive cells remarkably increased in PALS and RP(P<0.01). Conclusion: The distribution of T-lymphocyte changes in regenerated spleen and is benefit to inhibit the PAIgG.