磷酸钙钠/β-磷酸三钙陶瓷支架接种骨髓基质细胞成骨性能的研究

Bone formation by seeding bone marrow stromal cells on the sodium calcium phosphate/beta tricalcium phosphate scaffold

目的:观察磷酸钙钠／β-磷酸三钙(NaCaPO4/β-TCP)支架接种骨髓基质细胞后植入裸鼠皮下的成骨性能。方法:通过理化方法,将煅烧牛松质骨转化为NaCaPO4/β-TCP双相钙磷陶瓷。获取兔髂骨松质骨骨髓基质细胞,体外分离、扩增、诱导后,接种于NaCaPO4/β-TCP支架。将支架／细胞复合物植入裸鼠背部皮下,NaCaPO4/β-TCP陶瓷单纯植入作为对照。植入后4、8周取材,通过大体、组织学观察,评价成骨活性。结果:支架／细胞复合物植入后4周,材料表面见相对较成熟的骨组织,内部主要为软骨;植入后8周,大量骨小梁形成。可见骨髓腔、骨髓细胞及脂肪细胞,在支架材料和骨组织的邻接区域见成骨细胞及破骨细胞。可见软骨内成骨方式。结论:NaCaPO4/β-TCP支架接种骨髓基质细胞后显示良好的成骨活性,能够促进未成熟骨矿化,可以作为骨组织工程支架材料。

PURPOSE: To observe the osteogenesis of sodium calcium phosphate/β-tricalcium phosphate scaffold loaded with marrow stromal cells in a nude mice subcutaneous model. METHODS: To transform the sintered bovine can-cellous bone into NaCaPO4/β-TCP biphasic calcium phosphate ceramic by physical and chemical methods. Bone marrow stromal cells(BMSCs) were obtained by harvesting the cancellous bone from the iliac crest of a rabbit. Then the BMSCs were isolated , expanded and induced in vitro and seeded in the NaCaPO4/β-TCP ceramic scaffolds. The scaffold/BMSCs composites were implanted subcutaneously into the backs of nude mice. Implantation of NaCaPO4/β-TCP ceramic alone was acted as control. At 4 and 8 weeks after implantation, the specimens were harvested and the osteogenetic activity were evaluated by gross and histologic observation. RESULTS: At 4 weeks after implantation, the relatively mature bone were seen on the surface of the material, the inner part of the material were mainly cartilaginous bone . At 8 weeks after implantation, A large amount of trabecula bone had formed and the medullary cavity , medullary cells and lipocytes could be seen. Osteoblasts and osteoclasts were seen between the scaffolds and the local bone in the transition area. The endochon-dral bone formation pattern were observed. CONCLUSIONS: The NaCaPO4/β-TCP scaffold/BMSCs composites showed good osteogenetic activity and could promote mineralization of the immature bone. It can be used as the bone tissue engineering scaffolds.