rhBMP-2及rhbFGF对兔骨髓间充质干细胞碱性磷酸酶表达的影响

The long-term effects of rhBMP-2 and rhbFGF on alkaline phosphatase of bone mesenchymal stem cells

目的:检测rhBMP-2和rhbFGF单独、联合及序贯作用对兔骨髓间充质干细胞碱性磷酸酶作用的长期效应。方法:在细胞培养技术下,采用碱性磷酸酶检测试剂盒检测单独、联合及序贯施加rhBMP-2和rhbFGF后,兔骨髓间充质干细胞在第2、5、7及10天时碱性磷酸酶的活性水平,以反映对细胞分化作用的影响,并进行统计学分析。结果:rhBMP-2能长期显著地促进细胞的碱性磷酸酶活性,而rhbFGF则起到抑制作用,且均呈剂量依赖性。rhBMP-2单独作用与2种生长因子联合作用的效应相近,且均高于rhbFGF单独作用或2种因子序贯作用的效应。结论:不同生长因子的不同应用方式.对骨髓间充质干细胞分化作用的长期效应不同。

PURPOSE: To evaluate the long-term effects of rhBMP-2 and rhbFGF on alkaline phosphatase (ALP) of rabbit bone mesenchymal stem cells by using them alone, associatedly and sequentially. METHODS: Under cell culture technique, ALP kits were used to evaluate ALP level of bone mesenchymal stem cells so as to reflect differentiation of the cells on the 2nd, 5th, 7th, and 10th day by adding rhBMP-2 and rhbFGF on different ways. RESULTS: Statistical analysis showed that rhBMP-2 could enhance while rhbFGF could inhibit the ALP level of rabbit bone mesenchymal stem cells significantly in long term, and also in a dose-dependent manner. The effects of using rhBMP-2 alone was similar to that of using the two growth factors associatedly, and they were significantly higher than that of using rhbFGF alone and using them sequentially. CONCLUSION: Different growth factor has different long-term effect on differentiation of bone mesenchymal stem cells by adding them on different ways.