慢性支气管炎大鼠IL-8水平与炎症细胞变化特点的探讨

A Study on Interleukin-8 Level and Inflammatory Cells Change in Rat Chronic Bronchitis

目的 探讨慢性支气管炎的气道炎症特点及IL -8在其发病中的作用。方法 以脂多糖 (LPS)气管内注入法复制慢性支气管炎大鼠模型。对各组大鼠支气管肺泡灌洗液 (BALF)进行细胞计数和分类 ,用酶联免疫标记法测定各组大鼠血清及肺组织匀浆液中IL -8水平 ,并观察其支气管肺组织病理形态学变化。结果 模型组BALF中白细胞总数及中性粒细胞数较正常组显著增高。模型组血清和肺组织匀浆液IL -8水平较正常组显著增高。结论 中性粒细胞和IL

Objective To explore the features of the airway inflammation in a rat model of chronic bronchitis and study the role of IL-8 in the pathogenesis of chronic bronchitis. Methods The rat model of chronic bronchitis was set up by injecting LPS into trachea. Cell count and cytological classification in BALF were performed in experimental group and control group. The level of IL-8 in both serum and lung tissues of the two groups rats was measured by ELISA. Results The levels of IL-8 in both serum and lung tissues in the rats with chronic bronchitis were significantly higher than those in healthy control rats.The number of neutrophil in BALF of the rats with chronic bronchitis was significantly more than that in healthy control rats. Conclusion Both IL-8 and neutrophil were involved in airway inflammation of chronic bronchitis, and might play an important role in the pathogenesis of chronic bronchitis.