快速埋线对偏头痛大鼠模型脑干组织G蛋白含量的影响

Effect of catgut fast embedding therapy on the content of G protein in brain stem of rats with migraine

目的:研究快速埋线对偏头痛大鼠模型脑干组织G蛋白α亚基含量的影响,并探讨其机制。方法:将40只健康雌性SD大鼠随机分成正常对照组、生理盐水组、模型对照组和快速埋线治疗组各10只,通过皮下注射硝酸甘油(10mg/kg)建立实验性偏头痛大鼠模型,模型对照组只造模,不作其他处理。快速埋线治疗组造模后30min,取太冲、风池穴作快速埋线治疗。实验结束后运用免疫印迹法(Westernblot)检测各组脑干组织抑制性G蛋白(inhibitoryGTP-bindingprotein,Gia)和刺激性G蛋白(stimulatingGTP-bindingprotein,Gsa)的含量。结果:动物造模后30min左右,均出现外耳发红、前肢频繁搔头,爬笼次数增多,烦躁不安现象。快速埋线治疗组动物在快速埋线治疗后,上述症状逐渐消失;模型对照组动物上述现象持续3h,继而出现倦卧,活动减少状态。4h后造模组大鼠脑干组织Gsa蛋白含量明显升高快速埋线治疗组第1,2条带分别为(175.8±15.7)%,(166.8±13.5)%此为相对值无单位,模型对照组分别为(118.6±12.2)%,(112.5±8.1)%与正常对照组的100%,100%和生理盐水组的(105.7±6.9)%,(103.4±9.6)%比较差异有非常显著性意义(q=22.75～13.0,P<0.01),Gia蛋白含量快速埋线治疗组、模型对照组分别为(97.4±8.7)%,(71.4±7.5)%与非造模组的100和(97.5±7.8)

AIM:To explore the influence of catgut fast implantation on the content of G protein alpha subunits in the brain stem of rat models with migraine. METHODS:The rat models of migraine were established by using the subcutaneous injection of nitroglycerin(10 mg/kg),and then the rats were divided randomly into four groups of 10 rats in each:normal control group,saline control group,model control group and catgut fast implantation treatment group(study group).Rats in the model control group received no treatment.Thirty minutes after model establishment,fast embedding therapy at the taichong and fengchi acupoints was performed.After the experiment, the contents of inhibitory GTP-binding protein(Gia)and stimulating GTP-binding protein(Gsa)in brain stem were detected by using the Western blot method in all the groups. RESULTS:Thirty minutes after model establishment,redness of external ears,frequent scratch of head with forelimbs,increasing number of climbing the cage and dysphoria were observed in all the groups,while the symptoms mentioned above disappeared gradually in the study group after therapy,and those in the model control group lasted from 3 hours,then tired lying and reducing activities were observed.The Gsa contents in brain stem were obviously increased 4 hours after model establishment, band 1 and band 2 were(175.8±15.7)%and(166.8±13.5)%in the study group, and(118.6 ±12.2)%and(112.5±8.1)%in the model control group, very significantly different from those in the normal control group(100%and 100%)and saline group[(105.7±6.9)%and(103.4±9.6)%](q=22.75-13.0,P< 0.01).The Gia contents in the study group and model control group were(97.4±8.7)%and(71.4±7.5)%respectively, significantly different from those in the non model establishment group [100%and (97.5±7.8)%](q=22.88,11.86,P< 0.01),the ratio of Gsa/Gia was increased. The Gsa content of brain stem in the study group was very significantly lower than that in the model control group(q=17.17, 18.6,P< 0.01),while the Gia content was very significantly higher than that in the model control group(q=11.81,P< 0.01), and the ratio of Gsa/Gia was decreased. There was no significant difference in the contents of Gsa and Gia in brain stem between the normal control group and saline group(q=1.71,1.16,1.14, P >0.05). CONCLUSION:G-protein-linked signaling system plays an important role in the attack of migraine,and the G-protein-linked signal transduction pathway mediated by catgut fast embedding may be one of the important mechanisms for treatment of migraine.