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神经科学研究中绿色荧光蛋白报告基因的应用

Application of green fluorescent protein reporter gene in the neuroscience
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摘要 随着研究手段的进步,人们对神经学的研究更加深入。绿色荧光蛋白作为一种新型的报告基因,具有荧光性质稳定、对生物体无毒性作用、检测时不需底物的特点,在神经细胞的基因表达、蛋白定位的研究上优于其他报告基因,尤其适合于活体细胞功能与形态相结合的研究。
作者 崔岚巍 李玉荣 杨雷 曲莉辉 贾淑伟 田淑君
机构地区 哈尔滨医科大学生理学教研室
出处 《中国临床康复》 CAS CSCD 2004年第25期5358-5359,共2页 Chinese Journal of Clinical Rehabilitation
关键词 神经科学 绿色荧光蛋白 报告基因 荧光抗体技术 理化性质
分类号 R741 [医药卫生—神经病学与精神病学]
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参考文献18

  • 1[1]Prasher DC, Eckenrod VK, Ward WW, et al. Primary structure of the aequorea victoria green-fluorescent protein. Gene 1992; 111 (2):229 -33
  • 2[2]Heim R, Cubett AB, TsienRY. Improved green fluorescent. Nature 1995; 373:663 - 4
  • 3[3]Miyake S, Makimura M, Kanegae Y, et al. Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc Natl Acad Sci USA 1996; 93:1320 - 4
  • 4[4]Mignone JK, Kukekov V, Chiang AS, et al. Neural stem and progenitor cells in nestin-GFP transgenic mice. J Comp Neurol 2004; 9; 463 (3): 311 - 24
  • 5[5]Suzuki R, Watanabe J, Arata S, et al. A transgenic mouse model for the detailed morphological study of astrocytes. Neurosci Res 2003; 47(4): 451 -4
  • 6[6]Fujioka H, Suzuki M, Yamanouchi K, et al. Generation of transgenic rats expressing enhanced green fluorescent protein in gonadotropin-releasing hormone neurons. Reprod Dev 2003; 49(6): 523 - 9
  • 7吴乐,李辉,李云庆.大鼠延髓背角Ⅲ层深部内GFP基因重组病毒标记神经元的形态学特点[J].解剖学报,2002,33(4):337-341. 被引量：1
  • 8[8]Sunaguchi M, Nishi M, Mizobe T, et al. Real-time imaging of green fluorescent protein-tagged beta 2-adrenergic receptor distribution in living cells. Brain Res 2003; 12,984(1 - 2): 21 - 32
  • 9[9]Tamamaki N, Yanagawa Y, Tomioka R, et al. Green fluorescent protein expression and colocalization with calretinin parvalbumin and somatostatin in the GAD67-GFP knock-in mouse. J Comp Neurol 2003; 1,467 ( 1 ): 60 - 79
  • 10[10]Lavoie JK, Cassell MD, Gross KW, et al. Localization of renin expressing cells in the brain, by use of a REN-eGFP transgenic model. Physiol Genomics 2004; 15,16(2): 240 - 6

二级参考文献27

  • 1Farina KL, Wyckoff jB, Rivera J, et al. Cell motility of tumor cells visuaslized in living intact primary tumors using green fluorescent protein[ J ]. Cancer Res, 1998,58 : 2528-2532.
  • 2Jung S, Ackerley C, lvanchuk S, et al. Tracking the invasiveness of human astrocytoma cells by using green fluorescent protein in an organotypical brain slice model[J]. J Neurosurg, 2001, 94 : 80-89.
  • 3Margolin W. Green fluorescent protein as a reporter for macromolecular localization in bacterial cells[ J]. Method, 2000,20 : 62-72.
  • 4Tavare JM, Fletcher LM, Welsh GI. Using green fluoresent protein to study intracellular signaling[J]. J Endocri,2001,170:297-306.
  • 5Zhang G, Gurtu V, Kain SR. An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells[J]. Biochem Biophys Res Commun, 1996,227 : 707-711.
  • 6Peterson DL, Sheoilam PL, Brown WE, et al. Animal models for brain tumors: historical perspectives and future directions [ J]. J Neurosurg, 1994.80: 865-876.
  • 7Micci MA,Learish RD,Li H.Neural stem cells express RET,produce nitric oxide,and survive transplantation in the gastrointestinal tract.Gastroenterology 2001; 21(4):757-66
  • 8Doetschl F,Garoia-Verdugo JM,Alvarez-Buylla A.Cellular composition and three-dimensional organization of the subventricular germinal zone in the adult mammalian brain.JNeuroscience 1997; 17(13):5046-61
  • 9Mcdonald JW,Liu XZ,Qu Y,et o2.Transplanted embryonic stem cells survive,differentiate and promote recovery in injured rat spinal cord.Nature medicine 1999; 5(12):1410-2
  • 10Clarke DL,Johansson CB,Wilbertz J,et al.Generalized potential of adult neural stem cells.Science 2000; 288:1660-3

共引文献27

中国临床康复
2004年 第25期

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