移植修复脑功能损害种子细胞源研究:τ蛋白mRNA在脐血单个核细胞中的表达(英文)

Seed cell source study on the transplantation and repair of brain impairment: tau protein mRNA expression in cord blood mononuclear cells

背景:脐血单个核细胞可作为种子细胞源以移植修复不同原因所致脑功能损害,但能否表达神经元另一重要特有分子τ蛋白?目的:探讨脐血单个核细胞在培养前后及细胞因子诱导作用下,τ蛋白mRNA的表达。设计:随机对照研究。地点和对象:实验地点:华中科技大学同济医学院。采集健康新生儿脐血8份,每份80mL。干预:在细胞培养过程中,使用细胞因子:表皮生长因子(epidermalgrowthfactor,EGF)和碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)。主要观察指标:①细胞形态。②τ蛋白mRNA在脐血单个核细胞中的表达。结果:培养前,脐血单个核细胞胞体小呈圆形,培养后EGF+bFGF组细胞胞体较大,有粗长突起形成,未加细胞因子所培养细胞胞体较小,突起较短、较细,形似星形细胞。反转录聚合酶链式反应(reverse-transcriptpolymerasechainreaction,RT-PCR)检测未培养的脐血单个核细胞τ蛋白mRNA呈阴性而MAP2mRNA表达阳性,培养后二者均表达阳性,在EGF+bFGF作用下,阳性表达更明显。结论:脐血单个核细胞表达MAP2mRNA,经培养后同时表达τ蛋白mRNA,细胞因子能增强这种作用。

BACKGROUND:Cord blood mononuclear cells(MNCs) can be used as seed cell source to transplant and repair the brain impairment caused by different reasons.However,it is still uncertain if it can express another specific molecule tau(J) protein.OBJECTIVE:To explore the mRNA expression of τprotein in cord blood mononuclear cells before and after culture and with the induction of cytokines. DESIGN:Randomized case control study.SETTING and PARTICIPANTS:Experiment site: Medical College of Huazhong University of Science and Technology. Eight specimens of cord blood were collected form healthy newborns each of 80 mL.INTERVENTIONS:Cytokines were used during cell culture:epidermal growth factor(EGF),basic fibroblast growth factor(bFGF).MAIN OUTCOME MEASURES:①cellular morphology.②mRNA expression of MNCs in cord blood.RESULTS:The soma of cord blood mononuclear cell was small and round before culture.It turned big and formed thick and long cytodenrities in EGF+bFGF group after culture.The cell soma was smaller and had shorter and thinner cytodenrities which looked like astrocytes when cultured without cytokines.There was negative expression of τprotein mRNA and positive expression of MAP2 mRNA in uncultured MNCs.The expression of both τprotein mRNA and MAP2 mRNA were positive after culture when assaying by RT-PCR.The expressions had increased by induction of EGF+bFGF.CONCLUSION:There is expression of MAP2 mRNA of cord blood MNCs.It will also express τprotein mRNA after culture.Cytokines can increase this expression.