赛庚啶对家兔主动脉阻断所致脊髓损伤的保护作用

Protective effect of cyproheptadine on spinal cord injury caused by suprarenal aorta blocking in rabbits

目的:探讨赛庚啶对家兔肾下腹主动脉阻断所致脊髓缺血性损伤的保护作用。方法:24只家兔随机分为假手术组(A组)、缺血组(B组)和赛庚啶组(C组,每组8只。肾下阻断腹主动脉40min后松开,C组于阻断前)10min静注赛庚啶2mg/kg,继以2mg/(kg·h)持续输注至松开前10min。测定给药前、松开前及松开后60min兔血中丙二醛MDA含()量。术后观察动物后肢神经功能、肌电图(EMG)和脊髓形态学变化。结果:B组主动脉阻断及再通后血中MDA含量明显升高(P<0.01),C组动物阻断及再通后无明显变化。C组动物术后瘫痪数低于B组,术后神经功能评分及EMG检查显示C组脊髓功能损伤明显轻于B组。B组脊髓病理变化明显,可见大量神经元坏死,C组偶见神经元变性,病理学评分两组有明显差异。结论:赛庚啶对家兔主动脉阻断导致的脊髓缺血性损害有一定的保护作用,这一作用与赛庚啶抗过氧化反应及其他多种药理作用有关。

AIM:To evaluate the protective effects of cyproheotadine on the spinal cord ischemic injury caused by suprarenal aorta blocking in rabbits. METHODS:Twenty four rabbits were randomly divided into three groups:sham operation group(A),ischemia group(B) and cyproheptadine group(C),with 8 rabbits in each group.The infrarenal aorta was cross clamped for 40 minutes and then was loosened.In group C,venous injection with a bolus of cyproheptadine 2 mg/kg was given 10 minutes before cross clamping followed by a 2 mg/kg per hour infusion for 30 minutes at 10 minutes before loosening.The levels of malondialdehyde(MDA) in plasma were assayed before,during and 60 minutes after crossclamping.The nerve function and the eletromyograms(EMG) of hindlimb were evaluated after operation.The morphological changes of spinal cord was examined after operation. RESULTS:In the group B,the contents of MDA in plasma increased significantly when aortic cross clamping and declamping(P< 0.01),but the contents in the group C had insignificant changes.The neurologic deficits in the group B were more severe compared with the group C(ratio of paralysis,neurologic score and EMG).In the group B,more pathological changes and greater number neuronal death could be seen,but in the group C,neuronal death was occasional.There were significant differences between the group B and the group C in the pathological score. CONCLUSION:Cyproheptadine can protect spinal cord ischemic injury in rabbits to some extent,and this effect is related with its antioxidation and other pharmacological effects.